Growth of phage materials Mod3 Day1 Tuesday Nov 4, 2008

p3 p8 ] > 20 nm DNA p9p7 p6 900 nm 10^10th phage/ml of seawater 2700 copies BioMaterials: Let’s start with the biology

p3 p8 ] > 20 nm DNA p9p7 p6 900 nm 2700 copies BioMaterials: Let’s start with the biology

1.Infection 2.Amplification 3.Morphogenesis + release of progeny phage new M13 phage particles within 10’ post-infection 1000 phage/cell within the first hour BioMaterials: Let’s start with the biology

Parts of your experiment today Phage purification Harvest phage infected cells. Phage in SUPERNATANT! Concentrate by precipitation (+PEG/salt) Pellet may not be visible…have faith Plaque assay Serially dilute phage in water Mix with bacteria Add warm “top agar” and plate on LB--> 37° overnight Give remaining phage to Luis

BioMaterials: now for materials Oxidized (purple)Reduced (clear)

Part 1 of your experiment today Hydrolyze Iridium with citrate at 90° 4 hrs Generates Ir colloidal particles Next time will oxidize to form IrOx colloidal particles which can react with phage p8 You must wear safety glasses, gloves, lab coat to do this part of today’s experiment

FNT 1.Describe display technology 2.Choose research proposal (5 possible topics) or collaborative art/science option