Detection of Harmful Algal Blooms (HAB) Created By: Matthew Barboza Supervising Professor: Dr. Sungyong Jung Analog Mixed signal Integrated Circuits (AMIC)

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Presentation transcript:

Detection of Harmful Algal Blooms (HAB) Created By: Matthew Barboza Supervising Professor: Dr. Sungyong Jung Analog Mixed signal Integrated Circuits (AMIC)

Purpose Main Problem: Harmful Algal Blooms (HAB’s) posing a threat to environment and local population. Current detection methods o Manual Sampling and Testing o accurate analysis – small areas, time consuming, after the event o Aerial Scanning o large area scan – uncertainties, may not be real-time Proposed Solution: Submerged array of sensors to provide real-time, large area identification for early warning and treatment

In Situ Wireless Sensing Network (WSN) Qualitative and quantitative analysis. Easy to cover vast space and time. Real time and in situ monitoring. Early warning system. Microsystin sensors

Overview Background: o Harmful Algal Blooms o Immunoassays o Optical Electronics, laser-diodes and PIN photodiodes o Transimpedance Amplifiers Set-up Devices Progress

Harmful Algal Blooms (HAB) What is a HAB? o Sudden increase of marine microorganism population algae, bacteria Why is it “harmful” o Hypoxic conditions (little oxygen) o Toxins e.g. Microcystin LR produced by Microsystis Aerugenosa

Immunoassay A technique of identifying a substance by its ability to bind to an antibody. - Collins English Dictionary

Laser Diodes Emission of light due to electron-hole recombination Monochromatic Narrow beam

Photodiode Incident light causes electron-hole pairs Carriers are swept across depletion region Increase in reverse current Current proportional to incident light PIN, P-type, Intrinsic, N-type

Transimpedance Amplifier (TIA) Input current signal Ii, output voltage signal Vo Amplifier gain is Vo/Ii (ohms)

Immunoassay Antibody – Antigen Bonding Site Laser Diode Photodetector Amplifier Circuit Microcontroller Analog to Digital Converter Wireless Transmitter Digitally Controlled Pulsing Communication Protocol Memory

Devices FDS1010 – Photodetector – Thorlabs D7805I – Laser Diode – Digikey OPA380 – Transimpedance amplifier – Digikey TiWi5 – Wireless dsPIC33FJ128MC802 – Microcontroller – Mouser

Progress Microcontroller Configuration o 25.3 ns Instruction cycle (98.8% of 40 MIPS) o Timer Interrupt ( ~ 1 second) o Single, 10-bit, ADC conversion

K. G. Sellner et al., “Harmful Algal Blooms: Causes, Impact, and Detection” in Society for Industrial Microbiology, ‎ Copyright © 2003 EBSCO Publishing, DOI /s