Z-box Binding Factors in Light Signal-controlled Plant Growth and Development Sudip Chattopadhyay National Institute of Plant Genome Research New Delhi.

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Presentation transcript:

Z-box Binding Factors in Light Signal-controlled Plant Growth and Development Sudip Chattopadhyay National Institute of Plant Genome Research New Delhi National Institute of Technology, Durgapur &

Arabidopsis seedling development SkotomorphogenesisPhotomorphogenesis DarkLight

Photomorphogenesis COP1 COP1, a master negative regulator of photomorphogenesis, acts as ubiquitin ligase Tumor Suppressor Protein: p53 (Mammals) Nature (2004)

basicLzip HY5, a positive regulator of photomorphogenesis

ZBF1/MYC2 acts as a negative regulator of blue light mediated seedling development Col atmyc2-3 atmyc Hypocotyl length (mm) BL fluence rate (  mol/sec/m 2 ) Red lightFar-red light Blue light WT zbf1-1 (atmyc2-3) WT zbf1-1 (atmyc2-3) WT zbf1-1 (atmyc2-3) Blue light WT zbf1-2 (atmyc2-2) ZBF1/MYC aa

Pphysically interactions with COP1 In vitro binding: Two-hybrid:

Physically interactions with COP1 Co-localization:

Regulation of genes in an additive or antagonistic manner

ZBF1/MYC2 acts as a negative regulator of blue light mediated seedling development Red lightFar-red light Blue light WT zbf1-1 (atmyc2-3) WT zbf1-1 (atmyc2-3) WT zbf1-1 (atmyc2-3) Col atmyc2-3 atmyc Hypocotyl length (mm) BL fluence rate (  mol/sec/m 2 ) Blue light WT zbf1-2 (atmyc2-2)

Morphological defects in adult stage Flowering time Lateral root development WT zbf1-1

ABA (abiotic stress) WT zbf1-1 JA (biotic stress) Morphological defects in adult stage

Cloning and over-expression in tomato transgenic plants Transformed tomato plants