DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)

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Presentation transcript:

DNA microarray

Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification) Optics (signal detection) Electronics, physics Research Integration

Gene Expression Gene Discovery Genotyping (SNPs) Drug Discovery Diagnosis Sequencing/resequencing Disease Barcoding, Drug Susceptibility Crop Improvement Basic Research

Preparation of DNA solution for microarray PCR amplification PCR product purification 96-well plate Quality control Collections of ESTs, library clones... DNA printing solution 384-well plate Re-racking

Modified from Printing of DNA microarray

Modified from Each spot represents different gene/clone

Flowers Leaves AAAAA mRNA isolation DNA microarray probe preparation cDNA labeling with fluorescent dyes

Modified from Flower Leaf DNA microarray hybridization

Modified from Labeled DNA hybridizes to corresponding DNA/gene

Laser excitation Cy5: ~650 nmCy3: ~550 nm Image overlay No changes Flower up-regulated genes Leaf up-regulated genes

Cy3 labeled cDNA from etiolated seedlings Cy5 labeled cDNA from green leaves Arabdidopsis 11.5K microarray

Girke et al Detection of cross hybridization

Gygi et al Correlation between protein and mRNA levels

Plant functional genomics Basic mechanism of plant growth and development Application in agricultural biotechnology 增加農作物產量創造高營養價值之農作物創造具高適應力之農作物利用農作物作為生物反應器

DNA microarray and beyond….

Insertion site identification from knockout population Mahalingam and Fedoroff, PNAS (2001) 13,

Pollack et al DNA copy number detection

Iyer et al. Nature (2001) 409, DNA-protein interaction

MacBeath et al. (2000) Science 289, Protein-protein interaction (protein microarray)