Petri Dish investigation. agar A gelatinous material derived from certain marine algae. used as food for bacteria and as a thickener in many food products.

Slides:

Advertisements

Similar presentations

Toothpaste Brand vs. Bacteria in your mouth

Advertisements

Designing an experiment:

Lab procedures when handling micro-organisms Distribution of micro-organisms in nature.

How Long Does A Sterile Field Remain Sterile? by Rebecca A. Burns, SUNY Canton, Canton New York This experiment was conducted to show the likelihood of.

Each microorganism has an optimum growth temperature, which below it growth decreases reversibly and above it decreases irreversibly due to degradation.

Scientific Method A series of steps designed to solve a problem or learn more about the world around you.

BACTERIA By: Ben & Zach. Research Research Question and Variables How does compression affect the growth of bacteria? IV Regular tile Smashed tile DV.

STREAKING AN AGAR PLATE FOR ISOLATIONOF BACTERIAL COLONIES BACTERIAL COLONIES.

Sterile technique When handling E. coli and other bacteria it is essential that the live cultures do not become contaminated with other bacteria or fungi.

PHT 381 Lab# 4. A Culture medium:- ❊ An artificial preparation which contains the essential elements and nutrients needed by the m.o to grow. (most.

L8 – Keeping things sterile

Investigating how we can kill microbes Lesson Aims: To study how we can kill microbes and to safely set up an experiment to compare different disinfectants.

PHT 381 Lab# 4. A Culture medium:- ❊ An artificial preparation which contains the essential elements and nutrients needed by the m.o to grow. (most.

Culture Media Lab 2:.

G ROWING BACTERIA. The action of antibiotics and disinfectants can be investigated using cultures of microorganisms (populations of microorganisms that.

Aseptic Techniques Noadswood Science, 2012.

Growing Microbes L.O: To understand how microorganisms can be safely cultured in a laboratory Answers in your book: Antibiotics do not work on which type.

Pharmaceutical Microbiology

2A: Microscopy. Post Lab 2 is assigned today and due by the time your lab meets next. Pre Lab 3 will be available on Wednesday at 5 PM and is also due.

Bacteria Lab Problem: Where is the biggest variation of bacteria found within the school? Background: Bacteria are found _________ They grow on nutrient.

Ex. 7: Aseptic Technique – Inoculating Different Types of Media Key concepts: 1.Different types of bacterial cultures: broth, agar slant, and agar deep.

Classification of bacteria

PREDICTING THE GROWTH OF MICROORGANISMS. Objectives Students will Use sterile techniques to collect a sample Observe the growth of microorganisms on a.

Isolation technique with aseptic techniques to cultivate bacteria

Micro (2-1) TOTAL VIABLE COUNT Dr. Shahzad Ali Assistant Professor Department of Wildlife and Ecology UVAS, Ravi Campus, Pattoki.

General Microbiology Laboratory Sterile Technique & Pure Culture Concept.

Study the effect of industrial antibiotics on microbial growth.

Laboratory Procedure for bacterial transformation with pGLO It’s glowing.

Bacterial Growth. I. Determine in terms of population size. Nature there is a of organisms living together. Nature there is a of organisms living together.

Is your cell phone making you sick? By Desiree McWhorter.

Practical Part Microscopic Examination of Microorganisms Experiments Identification of MOs Different Staining Techniques.

Results of Sources of Contamination. Fungi Bacteria.

Organizing the way that we perform studies in Science!

Micro-organisms S1 Science Biological Sciences. What are germs? There are lots of different types of germs. Germs are all around us. Germs are bad for.

HONORS BIOLOGY LABORATORY SKILLS The Scientific Method.

Pure Culture Techniques

Inquiry Lab- Investigating Cheese “Microbiomes”

Introduction In natural environments, microorganisms usually exist as mixed populations. However, if we are to study, characterize, and identify microorganisms,

PURPOSE to discover the variety of bacteria on Westwood’s campus and on the human body to study the growth of organisms in a closed system A: B:

Experiment Questions Leaf Yeast. For what purpose did you use antiseptic wash solution in the investigation of the growth of leaf yeast on agar plates?

Aseptic Technique This is how we grow bacteria and moulds without contaminating our cultures or ourselves. It is also called ‘sterile technique’. You must.

Qualitative Observation A qualitative observation uses your 5 senses and includes descriptions of sights, sounds, smells, and textures.

Where can we find bacteria?

Lab #2 Maze Lab. Problem What variables can change the amount of time required to complete a maze?

Antibiogram realisation on a bacterial strain. Materials avalaible Swab Agar with bacteria Antibiotics disks Mueller Hinton culture medium Deposit plan.

بسم الله الرحمن الرحيم 140 MICRO LAB 5 : PURIFICATION OF MICROORGANISM.

Precautions that can be taken when handling micro-organisms

Bacterial identification plating streaking how to inoculate how to observe.

Inoculating a Petri Dish Procedure for Dry and Wet Swab.

Kickoff Question Assuming you like beef, how do you like your hamburger cooked-well done, medium, or rare? Why?

Aerosols Sneeze simulation method. Aerosols Suspension of a microbe containing particle Airborne pathogenic microorganisms Constitute a major hazard to.

Science Projects using Bacteria Aseptic Lab Technique The following guidelines are meant to assist you in following proper sterile technique during bacteria.

Microbial Biotechnology Reem Alsharief Lab 3. General Methods of Isolation and selection of Microorganism Microbial isolation: To separate (a pure strain)

Isolation of microorganisms

Bacterial Cultures *Bacteria grow best in warm, moist, dark areas that contain a lot of food. -When we culture bacteria, we provide them with this environment.

Cultivation and Isolation of Bacteria NAS: You’re never too old to streak! 1Lecturer: Mr M. Zivuku.

Basic Microbiology and Immunology Practical Course 2016.

Bacteria Cell Culture and Reproduction

Lab 4: Isolation of microbes from different environments

Bacteria Cultures Biotechnology II.

Lab procedures when handling micro-organisms

Aim: To prepare pure culture by streak plate method.

In your own words, define or explain science

Honors Biology Laboratory Skills

Introduction to Microbiology

Bellringer Please pick up the Bacteria Lab worksheet on the front table if have not done so already. Read through the entire lab. After you have read.

Lab procedures when handling micro-organisms

Section 2: Scientific Methods

Streaking an Agar Plate For

Scientific Method.

Presentation transcript:

Petri Dish investigation

agar A gelatinous material derived from certain marine algae. used as food for bacteria and as a thickener in many food products (carrageenan)

inoculate To introduce microorganisms or infectious material into a culture medium.

The “Clamshell” method Turn the dish upside down when streaking to avoid germs from the air landing on the dish.

streak To make lines method of inoculating to make sure “germs” get all over the plate

Sterile Free from live bacteria or other micro-organisms: a sterile operating area sterile instruments. autoclave

Shape of bacterial cells a)Cocci - round bacterial cells. (cox-eye). b) Bacilli - rod- shaped bacterial cells. c) Spirilli (corkscrew) - spiral-shaped bacterial cells A B C

QHEIC-the Scientific Method QuestionHypothesisExperiment Interpret data Conclusion

Variables Independent Variables The factor that is purposely changed EX: The different locations to be swabbed Dependent Variable The factor that you expect to change as a result of what you purposely changed. EX: the amount of bacteria that grows

Procedure 1) Record the numbers of your dishes on your data chart and make sure the dishes are divided into A,B, C and D. Each group will get 1 dish. 2) Go to your first location and use your swab to gently rub the location. Rub the location for 10 seconds. 3) Invert dish,open quickly and streak the plate with the swab. 4) Repeat for other locations after discarding swab. 5) Remember to write down the location on your sheet.

The constants and the control Constants The factors that are kept the same in all trials 1. Swabbing method 2. Swabbing time 3. Temperature of room Control the trial that is not exposed to the varying condition EX: section D