Lab Midterm Review

ID Parts of the microscope

Laboratory Safety

Ocular Micrometer Calibration

Know How to Weigh Agar

Know Agar Properties

Figure 3-11 Amoeba, a Sarcodine X212

Figure 3-12 Paramecium bursaria, A Ciliate (X528)

Figure 3-19 A Coccus

Figure 3-20 A Bacillus

Figure 3-29 Staphylococcus Arrangement

Figure 3-30 Streptobacillus Arrangement

Figure 3-24 A Spirillum

Negative Stain

Figure 3-38 Gram Stain of Staphylococcus epidermidis (+) and Citrobacter diversus (-). (X1300)

Figure 3-42 An Acid-Fast Stain Using the ZN Method

Spores

Spore Stains

Figure 3-60 Peritrichous Flagella

Wet Mount (Hanging Drop)

Know about Flaming Tubes

Autoclave vs Oven

MARGIN ELEVATION convexumbonateplateau flat raised raised, spreading edge flat raised margin growth into medium round irregularfilamentousrhizoid Whole Colony Smooth, entire rhizoid Irregular (erose) lobate filamentous

Figure 2-6 Opaque Center and Spreading Edge

Figure 2-7 Shiny and Umbonate

Figure 2-8 Irregular and Rhizoid

Figure 2-9 Punctiform Colonies

Figure 2-14 Swarming Growth Pattern

Thio Broth

Streak Plate

API 20E Test All Negative All Positive

API 20E Test

Litmus Milk Test

Litmus Milk

Motility Test

TSI Slants

Urea Test

Citrate

Nitrate Broth Before Reagents

Nitrate Broths After Reagents

Nitrate Broth After Zinc

Nitrate Broth Test

MR Test

VP Test

IMViC

MacConkey Agar