Susanne Vrtala, PhD Department of Pathophysiology Center for Physiology and Pathophysiology Medical University of Vienna, Austria Recombinant mite allergens.

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Presentation transcript:

Susanne Vrtala, PhD Department of Pathophysiology Center for Physiology and Pathophysiology Medical University of Vienna, Austria Recombinant mite allergens for diagnosis and immunotherapy in mite allergic patients

Groups of mite allergens

Isolation and characterization of Der p allergens Baldo & Donovan, 1988

Expression and purification of the clone 30- derived allergen

Diagnosis of mite allergy - with mite extracts, which contain a mixture of allergenic and non-allergenic components - extracts are standardized for Der p 1 and Der p 2 - some other important allergens are underrepresented in mite extracts - extracts do not allow a component-specific diagnosis Development of new diagnostic concepts with recombinant mite allergens

IgE-reactivity profiles to Der p allergens

Recombinant mite allergens for immunotherapy Production of hypoallergenic derivatives of mite allergens with reduced allergenic activity Immunotherapy with mite extracts - No patient-tailored therapy is possible - sensitization to new allergens is possible - Induction of severe anaphylactic side effects is possible

Patient 1Patient 2Patient 3Patient 4mean Der p 2 (aa 1-53) Der p 2 (aa ) Der p 2 Hybrid Der p IgG Abs induced with rDer p 2 derivatives inhibit patients‘ IgE binding to Der p 2 wildtype

- Identification of new mite allergens - A combination of Der p 1 and Der p 2 allows diagnosis of almost all mite allergic patients - Production of hypoallergenic derivatives of Der p 2 with reduced allergenic activity and retained immunogenicity Summary

Medical University of Vienna, Department of Pathophysiology, Division of Immunopathology Rudolf Valenta Group Immunotechnology Margit Weghofer Kuan-Wei Chen Anne Casset Wayne Thomas Telethon Institute of Child Health Research, Perth University of Western Australia Friedrich Horak Department of Otorhinolaryngology Medical University of Vienna Peter Valent Division of Hematology and Hemostaseology Medical University of Vienna Walter Keller Institute of Chemistry Karl-Franzens University of Graz Gabrielle Pauli Hôpitaux Universitaires de Strasbourg Allergy Unit National Health Service, Rome Adriano Mari Marianne van Hage Karolinska Institutet, Stockholm This work was supported by grants F1803, F1805, F1809 and F1815 of the Austrian Science Fund.