HPLC Introduction & Safety P. van Poppel – Field Support SpecialistMarch 2010

Resources Solvents Pump Sampler Sample Column Detector Accessories (Fraction Collector, Oven, Valves…) Data System

Available Pumps Pumps Current –210/218Isocratic, one pump one solvent, combine two to get a gradient system. –212LCMS pump of choice –SD1/SD2Dual piston, pulseless, isocratic pump. Obsolete –220Isocratic, single piston pump. –230Ternary pump with proportioning valves, single piston –240Quaternary pump, single piston

Available Pumps Interchangeable heads: 5/10/ 25/50/100/ to 200ml/min flow rate Easy maintenance Upto 8700psi rating Add pumps for gradients! 210/218 Analytical to Preparative Pump SD1 Preparative Pump Interchangeable 50, 200,500,800 mL/min pump heads Pulse-free flow from mL/min to 3200 mL/min

Available Samplers Samplers Current –410Mid-size, single tray, full, partial and user program –420Full-size, 8 segment interchangeable trays, full, partial and user program –430Full size (optional robotic arm), 96-well plates full, partial and user program. –Manual injector Obsolete –400Single tray, full loop injection Note: Cooling options are available in 410, 420, 430

Available Samplers 420 Autosampler 1ul to 1ml capacity Multi vial formats Manual Injector 410 Autosampler 1ul to 10ml capability 84 2ml vials 24 10ml vials Interchangeable Syringes 430 Autosampler 0.1ul to 1ml capacity 96/384 well plates

Available Detectors Detectors Current –325Single/dual wavelength UV/VIS –335Photodiode array - PDA –356RI detector –380/385 Evaporative Light Scattering detector

Available Detectors nm wavelength range Analytical - Preparative flowcells Peak Purity Spectral Calculator “On the fly” spectra Unlimited wav. extracts 335 Diode Array nm wavelength range Analytical - Preparative flow cells Benchmark Noise & Drift specs Patented Dual Path cell UV only option Upgradeable from single- dual wavelength 325 UV-Vis 380/385 ELSD No chromaphore required Universal response Full gradient and solvent compatibility No need for derivatisation Excellent baseline stability Complements other LC detectors 10 degree evaporation Patented Gas flow design Programmable LED Intensity 356 RI High sensitivity for non- absorbing molecules Superior peak shape Full Automation via Galaxie™ Low dead-volume flow-cell Active temperature compensation Long-Life LED

Available Detectors Detectors Obsolete –340single wavelength UV, D2 (Thermo) –350RI detector –355RI detector –363 Fluorescence (Hitachi D2 lamp) –370Electro chemical (Antec) –ELSD 2100 (/ice) –Hitachi L-2485 FL

Available Accessories Accessories Current –440Fraction Collector –500Column Valve Module –510Mistral oven, serial communication –530Column Valve Organizer –Various Rheodyne and Valco manual valves Obsolete –701Full size x-y movement fraction collector –704Half size x-y movement fraction collector –520Oven

Available Accessories 3 or 6 column selection 2 independent heater zones Diverter valves for column switching/back flushing and fraction collection 6 solvent selection for method development Utilised with Fusion AE for Automated Method development 500 Column Valve Module 530 Fluidics Module Utilised in tandem with LC ReSponder 10 collects,1 waste,1 recycle valve Blow fractions lines clear with N2 Configures multi-solvent inlets for pump Pneumatically driven for safety No moving parts 440 Fraction Collector Accurate, reproducible fraction collection Efficient sample collection Large fraction capacity Huge flow rate range Simple, intuitive software Compact footprint Optional Fume Extraction Kit