PRODUCTION OF 1,3-PROPANEDIOL THROUGH METABOLIC ENGINEERING Advisor: Wei-Shou Hu Speaker: Justice Akhuamhenkhun Ace Lai

Properties of 1,3-PD Molecular weight 76.09 Viscous and colorless liquid Miscible with water Density = 1.0597 g/ cm MP = -28; BP, between 210 to 212 (celsius degree) Unsaturated compound with hydroxyl functional group

Applications copolyesters, polymers, textile fibres, and carpets organic solvent ingredient of wood paints and anti-freeze chief ingredient manufacturing adhesives, laminates, terracota- as protective reagent

Why we use bioengineering Cheaper in cost Easier in designing pathway Greener to environment

Metabolic pathway

Metabolic engineering I nature of synthesizing of 1,3PD tpi gene dhaT and dhaB, gene encoding glycerol dehydratase and PDOR DAR1, GPP2 encoding 3- phosphate dehydrogenase and glycerol 3-phosphate phosphatase

Metabolic engineering II Redesigning of yqhD Use of NADPH instead NADH knock down of tpi to manipulate pathway more efficiently

Calculation analysis Yield, 1,3-propanediol at a rate of 3.5 g/L/h, a titer of 135 g/L, and a weight yield of 51% in D-glucosefed-batch 10 L fermentations.

Conclusion DAR1 and GPP2 cloned from Saccharamyces cerevisiae encoding 3- phosphate dehydrogenase dhaB, dhaT gene from Klebsiella pneumoniae encoding PDOR and glycerol dehydratase yqhD from E. coli can produce PDOR as well tpi manipulated via knockdown to resolve carbon flux PEP and ATP dependent glucose pathway modified In all, contribute to higher yield synthesis