Carl Zeiss MicroImaging AIS Solutions for Digital Pathology Andrew Lesniak Director, Product Management

Update Zeiss, Trestle, Clarient (ACIS) The Device technology group was acquired from Clarient Inc., by Carl Zeiss in March Combined installation base of 600+ Units worldwide. Full acquisition – including both the Trestle and the ACIS family of products New entity formed under Carl Zeiss – Carl Zeiss MicroImaging AIS Commitment to the technology portfolio, including: -Integration into the Carl Zeiss Support structure (worldwide). -Committed R&D investments for technology enhancement. -Interoperability with the existing Zeiss product portfolio; including Image Acquisition, Mirax™ Platforms, and advanced technologies such as the PALM MicroBeam platform for Genomic and Phenotype applications.

Light Microscopy macro Advanced Imaging MicroscopyDigital Pathology Solutions Classical Technologies ImagingLaserDigital Detection cellular molecular Technology Applications For Digital Pathology Information Management: Leading Computer Aided Diagnostics and Analysis AP Workflow and Educational Data Management Leveraging a Leading Product Portfolio for a Complete Pathology Imaging Solution

Scalable Product Constellation

Small footprint 20x Plan-Apo objective with 0.8 NA Image capture from barcode area w/ barcode identification Optional 40x Plan- Apo objective with 0.95 NA Choice of different camera adaptors (1x or 0.63x) Optovar option (1.25x and 1.6x) Fluorescence option Resolution range from 0.11 µm/pixel µm/pixel MIRAX Lab Automation Digital Pathology Solution

Zeiss Microscope Platform and Optics Configuration Full Robotic Telepathology functionality, including focal control, condenser settings Scalable to Digital Whole Slide Acquisition with user selectable Objective (4, 10, 20, 40, 60) Multi-focal plane imaging and robust focus technology ensures for robust imaging capability. Automated Slide Handling of 1, 4, 50 slides Fluorescence Multi-Channel Capable MIRAX Micro Robotic Digital Pathology Solution

Overlay  Extended Focus Real-Time Acquisition of image series from different focus positions Unique Scanning Advancements: MicroField Acquisition

 Multi channel Fluorescence Image acquisition in several individual fluorescence channels DAPI Overlay TexasRed FITC Scanning Advancements: Multi-channel Fluorescence Acquisition

Digital Pathology Analysis: Innovations with Imaging Tissue Scoring and Quantitative Analysis Membrane Stains Nuclear stains Integrated Optical Density Rare Event Detection Sentinel Lymph nodes Bone marrow Object Detection and Counting Nuclear Cell Counting, Micro-Vessel Density, Ploidy Tissue Micro Array Scoring

Red blood cells ICC-negative lymphoid cells ICC-positive lymphoid cells ICC-negative myeloid cells ICC-positive myeloid cells SizeBlue Content Brown Content smallnone/lowmedium/high small highnone/low small highmedium/high large highnone/low large highmedium/high Digital Pathology Analysis: Cellular Classification in Cytospin®

Workflow, Educational and Reference Materials  Virtual Lab creation- organization of Digital Images by context  Linking a slide to relevant accession, histological and pathological context information  Linking areas of interest on a slide to relevant teaching or lesion specific information  Preservation of compound related effects for future reference Digital Pathology Workflow: Enabling Scale

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