Ch 13: Meiosis and Sexual Life Cycles 2016. Chapter 13: Meiosis From Topic 3.1 Essential idea: Every living organism inherits a blueprint for life from.

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Ch 13: Meiosis and Sexual Life Cycles 2016

Chapter 13: Meiosis From Topic 3.1 Essential idea: Every living organism inherits a blueprint for life from its parents. Understandings: A gene is a heritable factor that consists of a length of DNA and influences a specific characteristic. A gene occupies a specific position on a chromosome. From Topic 3.2 Essential idea: Chromosomes carry genes in a linear sequence that is shared by members of a species. Nature of science: Developments in research follow improvements in techniques—autoradiography was used to establish the length of DNA molecules in chromosomes (1.8). Understandings: In a eukaryote species there are different chromosomes that carry different genes. Homologous chromosomes carry the same sequence of genes but not necessarily the same alleles of those genes. Diploid nuclei have pairs of homologous chromosomes. Haploid nuclei have one chromosome of each pair. The number of chromosomes is a characteristic feature of members of a species. A karyogram shows the chromosomes of an organism in homologous pairs of decreasing length. Sex is determined by sex chromosomes and autosomes are chromosomes that do not determine sex. Applications and skills: Application: Cairns’ technique for measuring the length of DNA molecules by autoradiography. Application: Comparison of diploid chromosome numbers of Homo sapiens, Pan troglodytes, Canis familiaris, Oryza sativa, Parascaris equorum.. Application: Use of karyograms to deduce sex and diagnose Down syndrome in humans. Guidance: The terms karyotype and karyogram have different meanings. Karyotype is a property of a cell—the number and type of chromosomes present in the nucleus, not a photograph or diagram of them. Genome size is the total length of DNA in an organism. The examples of genome and chromosome number have been selected to allow points of interest to be raised. The two DNA molecules formed by DNA replication prior to cell division are considered to be sister chromatids until the splitting of the centromere at the start of anaphase. After this, they are individual chromosomes. Aim 6: Staining root tip squashes and microscope examination of chromosomes is recommended but not obligatory From Topic 6.6 (further discussed in the Sexual Reproduction Mini-Unit of HL 1) Understandings: A gene on the Y chromosome causes embryonic gonads to develop as testes and secrete testosterone.

Chapter 13: Meiosis From Topic 3.3 Essential idea: Alleles segregate during meiosis allowing new combinations to be formed by the fusion of gametes. Nature of science: Making careful observations—meiosis was discovered by microscope examination of dividing germ-line cells (1.8). Understandings: One diploid nucleus divides by meiosis to produce four haploid nuclei. The halving of the chromosome number allows a sexual life cycle with fusion of gametes. DNA is replicated before meiosis so that all chromosomes consist of two sister chromatids. The early stages of meiosis involve pairing of homologous chromosomes and crossing over followed by condensation. Orientation of pairs of homologous chromosomes prior to separation is random. Separation of pairs of homologous chromosomes in the first division of meiosis halves the chromosome number. Crossing over and random orientation promotes genetic variation. Fusion of gametes from different parents promotes genetic variation. Applications and skills: Skill: Drawing diagrams to show the stages of meiosis resulting in the formation of four haploid cells. Guidance: Preparation of microscope slides showing meiosis is challenging and permanent slides should be available in case no cells in meiosis are visible in temporary mounts. Drawings of the stages of meiosis do not need to include chiasmata. The process of chiasmata formation need not be explained. From Topic 3.4 Understandings: Gametes are haploid so contain only one allele of each gene. Fusion of gametes results in diploid zygotes with two alleles of each gene that may be the same allele or different alleles. The two alleles of each gene separate into different haploid daughter nuclei during meiosis. From Topic 3.5 (further discussed in the Biotech Mini-Unit of HL 1 and reinforced in HL 2) Understandings: Clones are groups of genetically identical organisms, derived from a single original parent cell. Many plant species and some animal species have natural methods of cloning. Animals can be cloned at the embryo stage by breaking up the embryo into more than one group of cells. Methods have been developed for cloning adult animals using differentiated cells. Application: Production of cloned embryos produced by somatic-cell nuclear transfer.

Chapter 13: Meiosis From Topic 10.1 Essential idea: Meiosis leads to independent assortment of chromosomes and unique composition of alleles in daughter cells. Understandings: Chromosomes replicate in interphase before meiosis. Homologous chromosomes separate in meiosis I. Sister chromatids separate in meiosis II. Crossing over is the exchange of DNA material between non-sister homologous chromatids. Crossing over produces new combinations of alleles on the chromosomes of the haploid cells. Chiasmata formation between non-sister chromatids can result in an exchange of alleles. Independent assortment of genes is due to the random orientation of pairs of homologous chromosomes in meiosis I. Applications and skills: Skill: Drawing diagrams to show chiasmata formed by crossing over. Guidance: Diagrams of chiasmata should show sister chromatids still closely aligned, except at the point where crossing over occurred and a chiasma was formed. Aim 6: Staining of lily anthers or other tissue containing germ-line cells and microscope examination to observe cells in meiosis are possible activities. From Topic 10.3 (introduced in HL 1 but reinforced in HL 2) Nature of science: Looking for patterns, trends and discrepancies— patterns of chromosome number in some genera can be explained by speciation due to polyploidy (3.1).

Heredity and Variation Heredity- Passing of traits from one generation to another Variation- Inherited Differences among individuals of the same species. Genetics- The study of heredity and hereditary variation

Heredity Review Offspring acquire genes from parents by inheriting chromosomes. Allele-one specific form of a gene, differing only be a few bases and occupying the same locus. Genes- The length of DNA that codes for a protein. Chromosomes- Sections of DNA that contain Genes and other Associated DNA. Genome- collection of the organisms genes

Chromosome Structure Review

Types of Reproduction Asexual single parent genetically identical (clone) very rarely generate any differences; if so, from a mutation Sexual Two parents each gives half unique combinations variation!

Chromosomes Somatic cells have 46 chromosomes (23 from mom and 23 from dad). The two chromosomes that compose of the same length, centromere position, and staining pattern are called homologous chromosomes (aka homologous pairs).

Karyogram Homologous autosomes 1-22 Same loci Sex Chromosomes 23 rd pair Not same loci (males) Diploid (2n): 46 Two sets of chromosomes Haploid (n): 23 One set Gamete – haploid reproductive cell. Karyogram:

Human Life Cycles Fertilization: Union of two gametes (n) Zygote is formed (2n) Gametes (sex cells) are produced in gonads (ovaries and testes). Meiosis is the process by which the chromosome number is reduced.

Meiosis Overview: Has 2 parts Meiosis overview: Karyotype/ non disjunction project intro Meiosis I Meiosis II

Meiosis I Interphase I Growing, Replication, Prepration Prophase I (approx 90% of time) Synapsis occurs Tetrad forms Crossing over occurs (chiasmata) Centrioles move apart Nuclear membrane and nucleoli disappear

Meiosis I Metaphase I Chromosomes move to midline Independent assortment happens (Mendel) Segregation happens (Mendel) Anaphase I Homologous chromosomes (2 chromatids) move to opposite sides at the beginning of Anaphase I.

Law of Independent Assortment Independent assortment: Mendel proposed that states that allele pairs separate independently during the formation of gametes.

Law of Independent Assortment 2n = ___ Therefore n = ___ Fruit Fly Spermatogonium Or Oogonium Predict how many different way’s the homologous pairs. Can independently assort during Metaphase I?

Option 1Option 2 How many different gametes can be ultimately produced? 2n2n Law of Independent Assortment

Law of Segregation Law of Segregation: Mendel proposed that allele pairs separate during gamete formation. hill.com/sites/ /student_view0/chapter11/meiosis_with_crossing_over.html

Meiosis I Telophase one Cell either pinches forming a cleavage furrow or new cell plate forms Result: two haploid cells are produced from one diploid cell; these two haploid cells would enter Meiosis II for further division

Meiosis Overview2 Meiosis overview: Karyotype/ non disjunction project intro Meiosis I Meiosis II

Prophase 2 Metaphase 2 Anaphase 2 Telophase 2 4 haploid daughter cells formed (gametes) Just like the process of mitosis to separate the sister chromatids

Meiosis Application Meiosis produces gametes, which are haploid cells that are genetically different.

Random fertilization: Genetic Variations! A zygote produced by mating of a woman and man has a unique genetic identity. An ovum is one of approximately 8 million possible chromosome combinations (actually 2 23 ). The successful sperm represents one of 8 million different possibilities (actually 2 23 ). The resulting zygote is composed of 1 in 70 trillion (2 23 x 2 23 ) possible combinations of chromosomes. Crossing over adds even more variation to this.

Genetic Variations! Mutations throughout generations have produced many different alleles for a given genes