Properties of transformed cells. הפרדת תאים.

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Presentation transcript:

Properties of transformed cells

הפרדת תאים

Cloning

Interaction with Substrate 1.הדבקה סלקטיבית 2.ניתוק סלקטיבי 3.מטרקס סלקטיבי

Fig Flow Cytometry. Principle of operation of flow cytophotometer. Cell stream in D-PBSA enters at the top, and sheath liquid, also D-PBSA, is injected around the cell stream to generate a laminar flow within the flow chamber. When the cell stream exits the chamber, it cuts a laser beam, and the signal generated triggers the charging electrode, thereby charging the cell stream. The cell stream then breaks up into droplets, induced by the 15- kHz vibration transducer attached to the flow chamber. The droplets carry the charge briefly applied to the exiting cell stream and are deflected by the electrode plates below the flow chamber. The charge is applied to the plates with a sufficient delay to allow for the transit time from cutting the beam to entering the space between the plates

אפיון תרבית תאים לפי סוג התא שורות תאים או סמנים לרקמה סמנים ספציפים לתא טרנספורמציה

Karyotyping Chromosome staining

Isoenzyme analysis

Cytokeratin – Immunofluorescent staining

GFAP in Glial cells – Immunoperoxidase staining of glialfibrillary acidic protein

DNA fingerprinting

מורפולוגיה

Normal human Glial cells Undifferentiated Normal human Glial cells Differentiated

זיהום בתרבית רקמה שימוש בציוד למניעת זיהום מצע גידול סטרילי עבודה סטרילית

אינדיקציה לזיהום שינוי בתרבית התאים שינוי צבע לא צפוי ריח באינקובטור הופעת מוקדים של זיהום

סוגי המזהמים פטריות חיידקים וירוסים שמרים מיקופלסמה

חיידקים שמרים עובש מושבות מיקופלסמה ( לא בתרבית תאים ) Scanning electron micrograph of mycoplasma growing on the surface of cultured cells