Created by: Maryam Badakhshi, Shannon O’Keefe, Laura Poloni, Hasmita Singh.

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Presentation transcript:

Created by: Maryam Badakhshi, Shannon O’Keefe, Laura Poloni, Hasmita Singh

Overview 1) Background & Applications 2) Apparatus 3) Laser Safety

1. Background & Applications

Optical Tweezers Public image by RockyRoccon 2007 Highly focused laser beam is used to physically hold and move microscopic dielectric objects Can manipulate objects with noncontact and direct trapping Advanced Physics Laboratory for the Optical Tweezers apparatus involves the determination of optical trap stiffness of silica beads through various methods

Manipulation of Nanodevices (Tong et. al., Nano Letters, 2010) Alignment and rotation of a silver nanowire (Nam et. al., IJPEM, 2009) Four spheres trapped and rotated by linearly scanning with laser light

(Gross et. al., Methods in Enzymology, 2010) Isolation and Visualization of DNA Trapping of two beads “Catching” a Single DNA Molecule Force-Extension analysis of the trapped DNA Staining with Fluorescent Dye Protein-coated DNA region Fluctuations in DNA molecule (Gross et. al., Methods in Enzymology, 2010)

2. Apparatus

Main Components Very Dangerous! Safe!

Main Components

3. Laser Safety

Optical Trap Laser Characteristics 980 nm  Infrared range 330mW maximum power Collimated beam Class 3B laser Optical Trapping Kit, Thorlabs

Laser Classification Class 3B Laser Safety Interlocks Class 1 working environment Hazardous under direct and specular reflection, but not diffuse reflection Direct exposure to beam is an eye hazard Maximum power 500mW Considered incapable of causing injury

Hazards Diffuse reflections Invisible Most dangerous procedure, contact your TA/Instructor Eye injuries without laser safety glasses Stray Beams Beam Alignment Biological Effects

Cornea Focussing element Lens Fine focus Vitreous Humor Retina Image is projected from the cornea and lens Connection to brain through optic nerve Fovea Sharp vision Vitreous Humor Schematic of the eye by SurferSam Online.

Biological Effects Laser Light nm Focussed beam on retina Amplification of light by human eye: 10,000 Extremely large irradiance Dependent on exposure time Retinal Hazard Region Thermal Effects Overheating Retina burns Scars / blind spots in the field of vision Invisible light: damage may only be detected post-injury Severe damage may require surgery or transplant Depending on location of the burn, could permanently lose: Central vision Peripheral vision Elements of Laser Safety, Gary E. Myers

Laser Hazards Control Remove wristwatches or reflective jewellery Close and lock the room door Place “Laser Work in Progress” warning sign on door In case of an emergency, contact your TA/Instructor or UofT Campus Police Return the laser controller key when completed Turn off laser when changing samples Wear laser safety glasses AT ALL TIMES - Wavelength and Optical Density If someone unexpectedly enters, turn laser off Thorlabs Laser Safety Industries

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