Koch’ Postulates 병원균과 질병의 지속적 연관성 분리, 순수배양 건강한 개체에 접종시 동일한 증상 재분리 후 동일한 병원균 확인
Plant vs. Animal No antibody, T cell response No circulating cell Systemic signal through plant Disposable and replaceable parts in plant
Gene for Gene theory Avr : avr (avirulence) gene R : r (resistance) gene R * Avr = Resistance R * avr = Susceptible r * Avr = Susceptible R * avr = Susceptible
Signal Molecules ROD, reactive oxygen species NO, nitric oxide SA, salicylic acid JA, jasmoic acid C 2 H 2, ethylene
신호전달 후 HR, hypersensive response Phytoalexins PR, pathogenesis related proteins Cell wall reinforcing, H 2 O 2 Ca ++ Active oxygens SA
R and Avr interactions PAMP(pathogen associated molecular pattern) through TTSS(type III secretion system), Fls2(flagellin receptor) AvrRpm1 increases phosphorylation of Rin4 and decrease PAMP signaling AvrRpt2, protease cleaves Rin4 Rpm1 interact with Rin4 and detect change in Rin4
내병성 육종 Exogeneous genes - pathogen origin (M, Coat proteins, replicase) - plant origin (RIP) Endogenous genes - SAR - HR - PR - Signal transduction
내병성 분자육종 Engineering of R for broad spectrum Expression of R in an inducible manner - broad specificity of pathogens - multifactorial defense mechanism - only w/I infected tissue 내병성 육종의 한계 : 병원균의 진화