1. How many grams of sucrose (Mr 342) would you need in order to make 500 mL of a 5 mM solution? 2. Assume a 36% (w/w) solution of protein (Mr ) in water has a density of 1.09 g/mL. What volume would you need in order to prepare 500 mL 200 μg/mL solution?

g mL

3. Calculate pH of 0.02 M NaOH. 4. Calculate pH when 25 mL 0.16 M NaOH is mixed with 50 mL 0.1 M HCl 5. What is the pH of a solution that is 0.4 M Na lactate and 0.2 M lactic acid? (pK a lactic acid = 3.73)

6. Michaelis constant of the enzyme is 1 mM. At what concentration of substrate will be the rate of reaction half of the maximum rate? 7. The rate of reaction at maximum saturation of enzyme with substrate was 500 μmol.s -1. Michaelis constant of the enzyme is 1000 nM. What is the rate of reaction at substrate concetration of 1 μM?

6. 1 mM μmol.s -1