COURSE OUTLINE ERT 317Kejuruteraan Biokimia / Biochemical Engineering Semester 1 2015/2016

Nilai unit/ Number of unit: 4 Jenis kursus/ Course type: Teras / Core Prasyarat/ Prerequisite: ERT 106 Biochemistry Sinopsis kursus / Course synopsis:   This course focuses on the importance of bioprocess engineering elements such as enzyme technology, fermentation and bioconversion technology. Mathematical representations of enzymatic reaction as well as microbial systems are featured among lecture topics especially on kinetics study.

Hasil Pembelajaran / Learning Outcomes:   At the end of the course, students are expected to be: Ability to apply knowledge and solve engineering problem related in developing enzyme reactions based on its kinetics study and applied catalysis. Ability to identify, formulate, analyze and design solutions complex engineering problems using mathematics principles, natural sciences and engineering sciences for batch and continuous microbial systems, metabolic pathways and kinetics study. Ability to propose bioconversion technologies of microbial, plant and animal cell culture for the production of value-added product and defend it through verbal presentation in a group.

Senarai eksperimen / List of experiments:   Lab 1: To analyze the effect of enzyme and substrate concentration on enzyme kinetics study Lab 2: To analyze the effect of immobilization method on the retention of enzymatic activity Lab 3: To analyze the effect of environmental conditions on growth kinetics of S. cerevisae in batch process Lab 4: Effect of cell immobilization on dye removal by bioabsorbent Lab 5: To organize an open-ended experiment based on bioconversion technology approaches

Pendekatan pembelajaran / Learning approach:   (i) Lecture = 42 hours (ii) Laboratory = 10 hours (iii) Tutorial = 12 hours (iv) PBL = 9 hours

Sumbangan penilaian / Evaluation contribution:   Peperiksaan/ Examination: 70 % Mid Term Examinations = 20 % Final Examination = 50 % Penilaian Berterusan/ Continual Assessment: 30 % Quizzes/Assignment =10 % Lab report =10 % Integrated Project =10 %

Senarai buku teks dan rujukan / List of text books and references :   Shuler, M. L. and Kargi, F. (2014). Bioprocess Engineering: Basic Concept. 2nd Ed. Upper Saddle River, NJ: Prentice Hall PTR Rao, D.G. (2010). Introduction to Biochemical engineering. 2nd Ed. Tata McGraw Hill Education Private Limited Dutta, R. (2008). Fundamentals of Biochemical Engineering. New Delhi: Ane Books India Katoh, S. and Yoshida, F. (2009). Biochemical Engineering: A Textbook for Engineers Chemists and Biologists. Weinheim: Wiley-VCH Verlag GmbH & Co Nielsen, J., Villadsen, J. and Gunner L. (2011). Bioreaction Engineering Principles. 3rd Ed. New York: Springer Science+Bussiness Media

RANCANGAN MENGAJAR/LESSON PLAN Minggu/Week Kandungan Kursus / Course Contents (Panduan/Guidelines) Tugasan Bacaan/Reading Assignment Pensyarah/Lecturer Week 1 - 2 (7/09 – 18/09) CHAPTER 1: ENZYME KINETICS AND APPLICATIONS Chapter 1.1: Kinetics of Enzyme Catalyzed Reactions Introduction - Classification of enzymes according to the reaction catalyzed and how enzymes work. Enzyme kinetics - Mechanistic model for simple enzyme kinetics, determination of rate parameters for Michaelis-Menten kinetics; models for more complex enzyme kinetics; effects of pH and temperature and insoluble substrates Chapter 1 Mrs. Hafiza Shukor Week 3 (21/09 – 25/09) Chapter 1.2 : Applied Enzyme Catalysis Immobilized enzyme systems - Methods of immobilization, diffusional limitations in immobilized enzyme systems, electrostatic and steric effects in immobilized enzyme systems Enzyme production and application – Large scale production of enzymes, medical and industrial utilization of enzymes Week 4 - 5 (28/9 – 9/10) CHAPTER 2: MAJOR METABOLICS PATHWAY Introduction to metabolism and bioenergetics. Glucose metabolism: Glycolysis and TCA cycle, respiration. Metabolism of nitrogenous compounds and hydrocarbons. Overview of biosynthesis for small and macromolecules, anaerobic and autotrophic metabolism. Chapter 2 Week 6 - 7 (12/10 – 23/10) CHAPTER 3 : KINETIC OF GROWTH IN BATCH AND CONTINOUS CULTURES Chapter 3.1 : Kinetic of Growth in Batch Culture Quantifying cell concentrations, growth patterns and kinetics in batch culture, effects of environmental conditions on growth kinetics, heat generation by microbial growth. Chapter 3 Dr. Saleha

CHAPTER 3 : KINETIC OF GROWTH IN BATCH AND CONTINOUS CULTURES Week 8-9 (26/10 – 6/11) CHAPTER 3 : KINETIC OF GROWTH IN BATCH AND CONTINOUS CULTURES Chapter 3.2 : Kinetic of Growth in Continuous Culture Some specific devices for continuous culture, derivation for ideal chemostat, application of chemostat and its deviation of ideality. Chapter 3 Dr. Saleha Week 10 (9/11 – 13/11) Cuti Pertengahan Semester / Mid-semester Break Week 11 (16/11 – 20/11) CHAPTER 4: STOICHIOMETRY OF MICROBIAL GROWTH AND PRODUCT FORMATION Overall growth stoichiometry and design medium formulation, stoichiometric calculation of elemental balances and degree of reduction, theoretical predictions of yield coefficients. Chapter 4 Week 12-13 (23/11 – 4/12) CHAPTER 5 : BIOPROCESS CONSIDERATIONS IN USING ANIMAL AND PLANT CELL CULTURES Chapter 5.1 : Considerations in Using Animal Cell Culture Structure and biochemistry of animal cells, methods used for the cultivation of animal cells, products of animal cell cultures (3 hours) Chapter 5.2 : Considerations in Using Plant Cell Culture The importance and advantages of plant cell culture, plant cells in culture compare to microbes, methods used for the cultivation of plant cells, economics of plant cell tissue culture Chapter 5 Week 14 - 15 (7/12 – 18/12) CHAPTER 6: BIOCONVERSION TECHNOLOGIES FOR INDUSTRIAL BIOPROCESSES Type of bioconversion approaches e.g solid-state and liquid-state, advantages and disadvantages of bioconversion, important factors for bioconversion. Application of microbial, plant and animal cell culture in bioconversion technologies utilizing waste as substrate for production of value-added product. Chapter 6 Week 16 (21/12 – 25/12) Minggu Ulangkaji / Revision Weeks Week 17 - 20 (28/12 – 15/01) Peperiksaan Akhir Semester/ Final Examination

ERT319 Industrial Waste Treatment Taman Muhibah - Bilik Kuliah PPKBP   RK 28 - Year 3 8.00-9.00 9.00-10.00 10.00-11.00 11.00-12.00 12.00-1.00 1.00-2.00 2.00-3.00 3.00-4.00 4.00-5.00 5.00-6.00 6.00-7.00 ISNIN ERT319 Industrial Waste Treatment Taman Muhibah - Bilik Kuliah PPKBP UUW322 Thinking Skills Dragon - DKD 3 ERT318 / ERT317 Unit Operations / Biochemical Engineering LAB Dragon - Unit Operation Lab / Scale Lab SELASA ERT319 Industrial Waste Treatment Dragon - DKD 4 ERT316 Reaction Engineering Dragon - DKD 4 RABU ERT318 Unit Operations Dragon - DKD 5 ERT317 Biochemical Engineering Dragon - DKD 2 EQT271 Engineering Statistics Dragon - DKD 3 KHAMIS UUW235 Ethnic Relation Dragon - DKD 5 ERT316 Reaction Engineering Dragon - DKD 5 JUMAAT ERT317 Biochemical Engineering Dragon - DKD 1 EQT271 Engineering Statistics Dragon - DKD 1