 Accurately measure and transfer small amounts of liquid  Volumes commonly range from 0.5 – 2000  L (1/millionth of a liter aka )  2-20  L in lab today

 In a P20, the top number refers to 10’s of μl, the middle number refers to μl’s and the bottom number refers to 1/10ths of μl.

 Never draw liquid directly into shaft of the pipette – ALWAYS HAVE A YELLOW PLASTIC TIP IN PLACE  Firmly press the micropipette shaft straight down onto a plastic tip  When finished with the tip press the ejector button to remove the disposable tip

 There are two stops  Depress the plunger to the 1 st stop  With tip held vertically in liquid slowly allow plunger to rise back to the release position to draw to up correct volume  To dispense slowly depress to the 1 st stop, pause a second, depress to 2 nd stop – plunger is flush with top of shaft – withdraw the tip without releasing the plunger

 Place tip below the surface but not all the way to the bottom

 Using Micropipettes Set pipette volume only within the range specified for that micropipette.  Do not attempt to set a volume beyond the pipette's minimum or maximum values. This will damage the micrometer gears!

 When using a micropipette, first apply a tip. Forgetting to do this would ruin the precision piston that measures fluid volume.  Always keep a micropipette in a vertical position when there is fluid in the tip. Do not allow liquid to accidentally run back into the piston.

 Use your thumb to control the speed at which the plunger rises after taking up or ejecting fluid. Letting it snap back damages the piston!  There should be no snapping noises.

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