Lecture 6 ELISA!

E L I S A nzyme- inked mmuno- orbent ssay Protein that facilitates a chemical reaction Immune system - antibodies Survey technique: High throughput Overview

Antibodies? Virus H-bonding signature

Antibodies? Atrazine How can you use antibodies to detect it?

Antibodies? Expose Collect sera Harvest atrazine specific antibodies

How ELISA works Competition for the antibody active sites between: Analyte of interest - Antigen (ie. Atrazine) Analyte mimic or antigen mimic

The Major Players Compound of interest Atrazine =

The Major Players Competitor = E ELISA

The Major Players The Antibody ELISA Fe

Detection E H2O2H2O2 Compound that is detected BlueYellow via enzyme catalyzed reaction

Cross-reactivity AtrazineDesethyl Atrazine Simazine Ametryn