Single Particle Cryo-EM Wen Jiang Markey Center for Structural Biology

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Presentation transcript:

Single Particle Cryo-EM Wen Jiang Markey Center for Structural Biology

Cryo-EM: Imaging & 3-D Reconstruction Imaging (3D  2D) Reconstruction (2D  3D)

7Å 7Å 10Å 4Å 3Å 3.5Å 5Å 20Å P22 phage West Nile Virus T7 capsid II  15 phage T7 capsid I resolution T7 capsid I (asym)  15 phage (asym) Single Particle Cryo-EM: Jiang Purdue Circovirus (PCV2) Tulane Virus DNA nano-particles

Computing Needs for Cryo-EM Projection 2-D Alignment 3-D Reconstruction Image Processing Tasks Desktop Group of Desktops Cluster Condor Computing Resources ?

Cryo-EM on DiaGrid: Benefits pre-configured installation. As a user, you don’t need to worry about software download and installation. Just focus on your science projects. rapid dissemination of new algorithms. Newly developed image processing algorithms can be installed here to immediately benefit all user projects. large computing resource. The computational resources for image processing/3-D reconstruction tasks are provided at no cost to users by DiaGrid which leverages the vast amount of computing resources at Purdue and its partner institutions.

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