Characteristics Useful for the Diagnosis and Classification of Non-Lymphoid Neoplasms Gross Pathology Counts and Cytology Blood Bone Marrow Lineage Assessment Flow Immunophenotyping Cyto- and Immunohistochemistry Immune Receptor Gene Rearrangements Genetics Clonality Alterations in Oncogenes/Tumor Suppressors Gene Expression Patterns Biological Characteristics Disease Course Transplantation

Gross Pathology Organ Size, Color, Appearance Particularly Spleen, Liver, Lymph Nodes Color of Bone Marrow Presence of Inflammatory Lesions Presence of Non-hematopoietic Tumors

Counts and Cytology Peripheral Blood Total White Blood Cell Count White Blood Cell Differential Count including Presence of Young Forms (Blasts) Presence of Abnormal Leukocyte Morphology Hemoglobin Bone Marrow Nucleated Cell Differential Count Percentage Young Forms (Blasts) Presence of Abnormal Megakaryocytes, Erythroid Cells, or Granulocytes

Lineage Assessment of Young Forms and Accompanying Cells Immunophenotyping of Blood, Bone Marrow Cytochemical & Immunohistochemical Stains

Histopathology of Bone Marrow, Organs and Other Tissues Amount, Appearance, and Location of Myeloid Cells Disappearance of Normal Cell Types Disruption of Normal Architecture Spread into Adjacent Structures

Genetics Assessment of Clonality: Karyotype, Proviral Integrations, Immune Receptor Gene Rearrangements Alterations in Oncogenes/Tumor Suppressor Genes Gene Expression Patterns

Biological Features-Disease Course Time from Detection of Illness by Observation and Physical Examination Time from Detection of Illness by Peripheral Blood Abnormalities

Biological Features- Transplantation Variables include Number of cells injected Route of injection Character of disease in recipient (localized vs. disseminated) Type of recipient Immunodeficient, Nude Immunodeficient, Other Histocompatible, lethally irradiated Histocompatible, sub-lethally irradiated Histocompatible, unirradiated Time from transplantation to illness