BY: ASIA HILLIARD SAM MCMILLAN The Effect of Alcohol Chain Length on Psuedomonas Fluorescens Lag Phase

Introduction Pseduomonas fluorescens: a gram negative, rod- shaped bacteria that is well-adapted to living in soil, water, and plant surface environments.

Preliminary Experiment

Question & Methods Question: What is the effect of alcohols of varying carbon chain length on the lag phase of Pseudomonas fluorescens? Method  Various alcohols  Various concentration of alcohol  Low salt Luria broth  Distilled water

Results

y = x R² =

Conclusion The carbon chain length of alcohol is directly proportional to an increase in Pseduomonas fluorescens lag phase. Future Questions:  To what extent does the trend line hold true?  What causes propyl alcohols to have a different effect on Pseudomonas fluorescens lag phase?

References Picture Sources Pugachev, Mikhail V.; Shtyrlin, Nikita V.; Sapozhnikov, Sergey V.; et al.Source: BIOORGANIC & MEDICINAL CHEMISTRY Volume: 21 Issue: 23 Pag es: DOI: /j.bmc Published: DEC Yang, Shan; Zhang, Zhiqing; Wang, Fang; et al.Source: JOURNAL OF POLYMERRESEARCHVolume: 20Issue: 12 ArticleNumber: 307 DOI: /s Published: NOV Lee, Soo Youn; Lee, Seonyoung; Lee, Jiho; et al.Source: MATERIALS LETTERS Volume: 110Pages: DOI: /j.matlet Published: NOV utility.org/image/gallery. jsp?title=Alkanols utility.org/image/gallery. jsp?title=Alkanols logs/cw/2009/12/18/che mistry-worlds-weekly- round-up-of-money-and- molecules-98/ logs/cw/2009/12/18/che mistry-worlds-weekly- round-up-of-money-and- molecules-98/ Resources