 -amanitin CHX 1 2 3 4 5 6 7 8 0 2 4 6 0 2 4 6 (h) K921 Ac-Abl Abl Additional file, Figure S4.

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 -amanitin CHX (h) K921 Ac-Abl Abl Additional file, Figure S4

BAA+1 B ions+1 Y ions+2 B ions+2 Y ionsY 1 L K P A P P P P P A C Me T G K Ac Suppl. Fig. 1B. MS/MS of the peptide ion at m/z 477.8, corresponding to the (M+3H)3+ species with a mass of 1431Da.

p300 p300 -/- HCT IR (5Gy) K921 Ac-Abl Abl Suppl. Fig. S2. p300 HAT is dispensable for IR-induced K921 acetylation. HCT116 and HCT116/p300 - cells wre either mock-treated or IR (5 Gy) exposed and harvested 4 h post-treatment. Lysates were adjusted for equal protein content and probed with K921 acetyl-Abl (top panel), anti-Abl antibody (middle panel) or anti-p300 antibody (bottom panel).

 -amanitin CHX (h) K921 Ac-Abl Abl Additional file 3, Figure S3