EE Slawson Tempel, © WUSTL. STOP EE Slawson Tempel, © WUSTL.

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Presentation transcript:

EE Slawson Tempel, © WUSTL

STOP EE Slawson Tempel, © WUSTL

STOP EE Slawson Tempel, © WUSTL

OH STOP EE Slawson Tempel, © WUSTL

OH STOP EE Slawson Tempel, © WUSTL

STOP EE Slawson Tempel, © WUSTL

STOP EE Slawson Tempel, © WUSTL

STOP EE Slawson Tempel, © WUSTL

STOP EE Slawson Tempel, © WUSTL

STOP OH EE Slawson Tempel, © WUSTL

OH STOP EE Slawson Tempel, © WUSTL

OH STOP EE Slawson Tempel, © WUSTL

STOP EE Slawson Tempel, © WUSTL

STOP EE Slawson Tempel, © WUSTL

STOP EE Slawson Tempel, © WUSTL

STOP EE Slawson Tempel, © WUSTL

STOP OH EE Slawson Tempel, © WUSTL

G…G… © Illumina, EEST, © WUSTL

GC…GC…

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Flowcell 8 lanes For picture taking: Each lane is broken up into 100 tiles, each fluor is imaged separately – 2400 pictures taken per cycle Camera time is the limiting step! EE Slawson Tempel, © WUSTL

STOP Chemistry problem 1: terminator is retained EE Slawson Tempel, © WUSTL

OH Chemistry problem 2: fluor is retained EE Slawson Tempel, © WUSTL

STOP Chemistry problem 2: fluor is retained EE Slawson Tempel, © WUSTL

STOP Chemistry problem 2: fluor is retained EE Slawson Tempel, © WUSTL