Metabolomics and analytical chemistry: GC & GCMS Simone Bossi Analytical Chemistry Lab – Plant Physiology – Plant Biology.

Slides:



Advertisements
Similar presentations
Gas Chromatography.
Advertisements

Gas Chromatography, GC L.O.:  Explain the term: retention time.  Interpret gas chromatograms in terms of retention times and the approximate proportions.
AP CHEMISTRY. Review SI system -know prefixes in blue on pg 10 Mass vs weight-an object’s mass reflects the amount of matter it contains. An object’s.
Mobile phase is a gas! Stationary phase could be anything but a gas
Analysis of Residues. Laboratory Analysis of Debris and Other Samples 1. Preparation of Liquid Samples: Liquid samples are simply drawn into a special.
Gas Chromatography in the detection of Volatile Organic Compounds.
Chromatography CHEMISTRY Chromatography Chromatography is a technique for separating species based on physical or chemical properties. Usually.
In carbon-13 NMR, what do the number of peaks represent?
Analysing the METABOLOME 1.Metabolite Extraction 2.Metabolite Separation 3.Metabolite detection (with or without separation) 4.Data analysis.
Chromatography Russian scientist Tswett in 1906 used a glass columns packed with finely divided CaCO3 to separate plant pigments extracted by hexane. The.
Intro to Chromatographic Separations Chap 26. Originally based on separation and identification by color Originally based on separation and identification.
An introduction to chromatography. To identify the compounds of a mixture = qualitative analysis To quantify these compounds To retrieve the separated.
Chromatography and Instrumentation. Invented by a Russian Botanist Mikhail Tswett in 1903 He used chromatography to separate the colour pigments in plants.
Gas Chromatography And Mass Spectrometry
Chromatography Is a technique used to separate and identify the components of a mixture. Works by allowing the molecules present in the mixture to distribute.
Types of Chromatography Liquid chromatography versus gas chromatography? Applications? Volatile  GC Non-volatile  LC.
Chromatography Year 12.
Filtration and Evaporation Distillation Paper Chromatography SEPARATION TECHNIQUES.
Forensic Drug Analysis
GCMS: gas chromatography and mass spectrometry
By, Blessy Babu. What is Gas Chromatography?  Gas spectroscopy is a technique used to separate volatile components in a mixture.  It is particularly.
5 -1 FORENSIC DRUG ANALYSIS Drug Identification The challenge comes in selecting analytical procedures that will specifically identify a drug. This.
Chemical Analysis. Analytical Techniques When chemical evidence is collected at a crime scene, it must be run through an instrument. These instruments.
Chemical Ideas 7.6 Chromatography. The general principle. Use – to separate and identify components of mixtures. Several different types - paper, thin.
Chromatography Tomáš Mlčoch Pavel Moťka. Chromatography Described by Tswett in 1906 Described by Tswett in 1906 He separated some pigments using a tube.
Magnet Analytical Chemistry Unit 4
Separation Science Differences in IMFs can be used to separate chemical substances for further analysis. Differences in IMFs can be used to separate chemical.
GC Advantages 1. Very Large N (Very Long Columns) 2. No Packing Material (A=0) 3. Simple Mobile Phase (Compressed Gas) 4. Universal Detectors (FID) 5.
TECHNIQUES INVOVED IN PROTEOMICS,GENOMICS,TRANSCRIPTOMICS…….
Gas Chromatography Experiment. Gas Chromatography - Gas Chromatography (GC) is a common technique used to separate and identify volatile organic compounds.
Lecture 7 Chromatography Intro!.
Methods of Chemical Analysis. Selecting an Analytical Technique  Organic vs. Inorganic materials  Organic:  Inorganic:  Quantitative vs. Qualitative.
By- Bhavya, Harsh, Harshvardhan, Namrata, Ronit and Vidhatri
ERT 207 ANALYTICAL CHEMISTRY Alina Rahayu Mohamed PPK Bioproses Universiti Malaysia Perlis.
1 Gas Chromatography Lecture Liquid Stationary Phases In general, the polarity of the stationary phase should match that of the sample constituents.
Hydrolysis of Proteins and Chromatography
State that the mobile phase may be a liquid or a gas.
INTRODUCTION TO CHROMATOGRAPHY DR. Harrizul Rivai, m.s.
Dr Saleha Shamsudin. 1.INTRODUCTION Topics to be covered: 1.1 INTRODUCTION TO METHODS IN ANALYTICAL CHEMISTRY 1.2 STEPS IN QUANTITATIVE ANALYSIS.
Chemistry 2412 L Dr. Sheppard
Gas Chromatography Refractive Index Polarimetry Next Lecture: Lecture Problem 8 Due Next Thursday: Quiz 7 in Lecture Next Week’s Office Hours: Tuesday.
Ch 21 – Principles of Chromatography and Mass Spectrometry Ch 22 – Gas and Liquid Chromatography.
Separation Techniques
Gas Chromatography An Introduction. What is it? Gas chromatography – (gas-liquid chromatography) involves a sample being vaporized and injected onto the.
HPLC.
By Susan McCullough With Thanks to Lori Olson at SRI International.
Instrumental Analysis
Organic Analysis Basic concepts. Elements and Atoms Fundamental building block of all substances is the element. Fundamental building block of all substances.
1 Forensic Science Questioned Documents Devices. 2 Getting Started )A ransom note has been delivered to a family of a missing child. )How would the crime.
Lab Tools of Forensic Scientists. Detection Presumptive tests ◦ Give idea as to what probably is in a sample/what definitely is not in a sample  Ex:
Gas Chromatography Gas Chromatography Effendy De Lux Putra.
Drug detection and analysis
C1 Air quality IC1.1 Chromatography Learning Objectives: To know how chromatography works. To be able to calculate R f values for paper chromatography.
Presented By Swambe Chemicals , III Floor, Prayosha Complex,Chhani JakatNaka,Vadodara Gujarat, India
From the Greek for “color writing”
Physical Evidence and Organic Analysis
Chapter 5 – Organic Analysis
LU 3: Separation Techniques
Organic Instrumentation
SEPARATION TECHNIQUES
Forensic Drug Analysis
Microbiome: Metabolomics
What is chromatography?
Chromatography Daheeya Alenazi.
Paper title-Analytical techniques in chemistry
Microbiome: Metabolomics
GAS CHROMATOGRAPHY.
SEPARATION TECHNIQUES
Presentation transcript:

Metabolomics and analytical chemistry: GC & GCMS Simone Bossi Analytical Chemistry Lab – Plant Physiology – Plant Biology

Metabolomics is the "systematic study of the unique chemical fingerprints that specific cellular processes leave behind" - specifically, the study of their small- molecule metabolite profiles. B. Daviss, "Growing pains for metabolomics," The Scientist, 19[8]:25-28, April 25, 2005 Metabolome Proteome Transcriptome Genome

Analytical methods able to qualify and quantify single molecules or a class of metabolites. Colorimetry, Spectrophotometry, Electrophoresys, Immunology, Radiometry, Mass Spectrometry, NMR, Crystallography,…

CHROMATOGRAPHY Separate complex mixture coming from biological sample. CLASSIFICATION Mobile phase, stationary phase classification Shape of the support on which the stationary phase is fixed Operating conditions Chemistry or stechiometry of molecules with the phases …

Gas chromatography Volatile organic compounds Hydrophobic molecules extracted or dissolved in organic solvents Small molecules weakly polar Big and hydrophobic molecules volatile at high temperature

Liquid Injection

SPME: Solid Phase Micro Extraction

SBSE: Stir Bar Sorptive Extraction

The Gas chromatographer

The Injection port

The Column

The Oven

Data Analysis

Mass Spectrum and Identification

Feedback: Privacy Policy Feedback
Do Not Sell My Personal Information
About project: SlidePlayer Terms of Service

© 2024 SlidePlayer.com. Inc. All rights reserved.