1 Advisor : Cheng-Hsin Chuang Advisee :Wan-Ting Su Department of Mechanical Engineering & Institute of Nanotechnology, Southern Taiwan University, Tainan, TAIWAN Date ﹕ 2012/04/06 Paper Survey A microwire sensor for rapid detection of Escherichia coli K-12 in fresh produce Innovate Food Science and Emerging Technologies 12 (2011) Sungkyun Kim,Lin Lu, Jae-Hyun Chung,Kyonghoon Lee,Yong Li,Soojin Jun
Outline Paper Survey Sensor Design Results and discussion Experimental Result Future work
Sensor Design Fig.1. ( a) Microwire sensing unit (b) Power supply and measurement equipments
Results and Discussion DEP force and electroosmotic flowCapillary Fig2.Regression curve for enumeration of E.coli K-12cells Fig3.Stepwise produres for detection of E.coli K-12
Simulation Result Fig 4. Sequential simulation of electric field strength
Experiment Results Fig. 5. FESEM image of E.coli K-12 cells captured on the microwire. (a)Without and (b) with AC field ﹒
Experiment Results Fig. 6. Optimization of the working frequency for detection of E.coli K-12 at 10 6 CFU/ml Fig.7. Fluorescence intensity of E.coli K-12 in PBS buffer when applied with 20V PP and 3MHz
Experiment Results Fig. 8. Selective capture of bactrial cells from E.coli K-12 internalized spinach solution: (a) FI values of E.coli K-12 in spinach and buffer solutions at different concentrations (b) FSEM image of the microwire sensor. Many spinach particles are captured on the wire surface
Outline Paper Survey Sensor Design Results and discussion Experimental Result Future work
Experimental MethodExperimental
Experimental Results Flow Rate ： 0.1 ml /hr Al 2 O 3 Concentration：2 wt% BSA Concentration：1 μM Applied Frequency：10 kHz Apply Voltage：10 Vpp Applied Time ： 0 min Applied Time ： 2 min Applied Time ： 16 min Applied Time ： 30 min
Outline Paper Survey Sensor Design Results and discussion Experimental Result Future work
Paper Survey Experiment by Different Parameter Experimental Sample Using Bladder Cancer Cell SEM Training
Thank you for your attention !!