Catheter-related blood stream infections. Objectives 1.Catheter-related blood stream infections - Present scenario 2.Methods of diagnosing CRBSI 3.Importance.

Slides:

Advertisements

Similar presentations

Jan Bazner-Chandler RN, MSN, CNS, CPNP

Advertisements

PART II CATHETER RELATED BLOOD STREAM INFECTION (CR-BSI) CATHETER RELATED URINARY TRACT INFECTIN (CR-UTI)

Central Line Bundle Education

MANUAL CATHETER ASSOCIATED BLOOD STREAM INFECTION (CABSI) SURVEILLANCE

Cerebrospinal fluid Culture + Body Fluid Culture.

Diagnostic Testing in the Microbiology Laboratory Jane Wong Public Health Microbiologist September 30, 2003

Dr. Elizabeth Bryce Vancouver Coastal Health Making Sense out of Medical Microbiology Reports.

URINARY TRACT INFECTION

Clinical Scenario  A 42 year old white female was admitted to the hospital with hematemesis and melena. The patient had a history of cirrhosis with ascites.

HAI Surveillance & Definitions In LTCF

Blood Culture. Bacteremia: Types  Transient: Disruption of mucosal surfaces (dental or surgical procedures)  Intermittent: Associated with abscesses.

DR. P. L. KASHYAP UNDER GUIDANCE OF: DR. ROHINI KELKAR DEPT. OF MICROBIOLOGY TATA MEMORIAL HOSPITAL, MUMBAI Automated methods in the Microbiology Lab-Issues.

Good Job!. Let’s continue this downward trend of reducing blood culture contamination in the ED!!! Here’s a quick review: Step 1 Verify Order. Only WRITTEN.

Welcome to MICROnesia 4 “Bug” Case Studies “Life of a Blood Culture” Slide Show Questions welcomed!

1 25th ECCMID , Munich, Germany Magnitude of bacteremia predicts one-year mortality Kim O. Gradel 1, Henrik C. Schønheyder 1,2, Mette Søgaard 1,

Comparison of the Diagnostic Value of the Standard Tube Agglutination Test and the ELISA IgG and IgM in Patients with Brucellosis Presented by Dr. Md.

Only 5-15% of blood cultures are (+) in febrile patients A.Types of bacteremia: Extravascular via the lymphatic's Intravascular: i.e. CVC infections B.Types.

MLAB 2434 –Microbiology Keri Brophy-Martinez

Only 5-15% of blood cultures are (+) in febrile patients. A.Types of bacteremia: Extravascular: via the lymphatic's. Intravascular: i.e. CVC infections.

Blood Culture بسم الله الرحمن الرحيم

1 Catheter-Related Bloodstream Infection Guidance Revisited Janice Pohlman, M.D. FDA AIDAC Presentation October 14, 2004.

Eunice Huang, MD, MS APSA Education Day Palm Desert, CA May 22, 2011

Catheter-Associated Bloodstream Infections Based on Infectious Disease Society of America guidelines Clinical Infectious Diseases 2001;32: Rey.

ESBL, MBL and Amp C beta lactamase detection DEPARTMENT OF MICROBIOLOGY JIPMER PUDUCHERRY.

Bacteremia and CRBSI as Labeled BSI Indications: A Regulatory History Alfred Sorbello, DO Medical Officer CDER/Division of Anti-Infective Drug Products.

Value of rapid antigen detection tests (RADTs) for diagnosis of invasive group A Streptococci (GAS) 3-centre, retrospective study: N=192 miscellaneous.

LAB 4: ASEPTIC TECHNIQUE AND ISOLATION OF BACTERIA

Infectious Disease Epidemiology, Module I Introduction.

© 2009 CSTS: The Cardiovascular Surgical Translational Study Using the Centers for Disease Control and Prevention’s National Healthcare Safety Network.

Specimen Collection and Processing

Blood Culture. Aim of the test :-  An etiological diagnosis of bacteremia by aerobic and anaerobic cultivation of the blood, with identification and.

Controls for Blood Septicemia Nucleic Acid Tests Mark Manak BBI Diagnostics, Inc. A Division of SeraCare Life Sciences, Inc. SoGAT XIX Meeting Berne, Switzerland.

Quality Control in Microbiology - 1 5%-30% of positive blood cultures represent contamination with skin To keep numbers of contaminants.

Processing of Clinical Specimen for Immunofluorescence staining & Viral culture Exercise II – Day 4 Virology Section Department of Microbiology JIPMER.

Quality Education for a Healthier Scotland Blood Cultures.

Hospital Acquired Catheter-Related Bloodstream Infections (CR-BSI)

REDUCING CATHETER ASSOCIATED URINARY TRACT INFECTIONS CLINICAL EXCELLENCE COMMISSION 2015 URINE COLLECTION, CULTURE and CATHETERISATION IN ACUTE SETTINGS.

Journal club Diagnostic accuracy of Urinalysis for UTI in Infants

Infection Prevention: Recognizing and Communicating CAUTI

APIC Greater NY Chapter 13 Journal Club Session November 18, 2015 by Steven Bock RN BSN CIC Infection Prevention and Control Department /

Gülden Çelik. Learning Objectives At the end of this lecture, the student should be able to: Define bacteremia, fungemia, and sepsis List the main types.

Medical University of Vienna Jeroen S. de Bruin Validation of Fuzzy Logic in Infection Surveillance Jeroen S. de Bruin, Alexander Blacky, Walter Koller,

Catheter-Related Blood Stream Infections A Phase 2 Randomized, Controlled Trial of Dalbavancin vs. Vancomycin Tim Henkel, MD, PhD Executive VP and Chief.

Jean‑Jacques Parienti, M.D., Ph.D., Nicolas Mongardon, M.D.,

1 Issues in Conduct of Catheter Related Infection (CRI) Studies Charles Knirsch, MD, MPH FDA Anti-infective Advisory Committee Meeting October 14, 2004.

Clinical Infectious Diseases 2012;55(6):764–70 Jan Vydra,1 Ryan M. Shanley,2 Ige George,1 Celalettin Ustun,1 Angela R. Smith,4 Daniel J. Weisdorf,1 and.

Routine replacement of CVC is not necessary JF Timsit MD.

Laboratory diagnoses of infections agents. DIFFERENT TYPES OF AND APPROACHES TO CLINICAL SAMPLE COLLECTION.

Preventing Vascular Access Device (DIV) Infection

Intravascular Catheter-Related Infection: Clinical Practice Guideline 감염 내과 박 기 호.

IM R4 박미나 Management of infected Central venous catheters used for hemodialysis.

Sending Samples Kim Wilson, Matron, Infection Prevention UHMB] 12/03/2012.

Prevention of Catheter Related Infections Dr.E.Shojaei T.U.M.S Jan 2016.

Sample collection & laboratory methods for identification bacteria

GIT-block Microbiology Lab.

Eleanor Bates, Colette McCambridge, Bob Philips, Jonathan Sandoe

MLSM 508 Blood Culture Systems (Manual System)

Central Line Infection: Pull or Leave it in

Detection of bacteria in blood products

How to discriminate contamination from bloodstream infection due to coagulase- negative staphylococci: a prospective study with 654 patients L. Elzi,

Recurrent catheter-related Rhodotorula rubra infection

A. Simon, U. Bode, K. Beutel Clinical Microbiology and Infection

Overview of catheter-related infections with special emphasis on prevention based on educational programs P. Eggimann, D. Pittet Clinical Microbiology.

How to discriminate contamination from bloodstream infection due to coagulase- negative staphylococci: a prospective study with 654 patients L. Elzi,

Brucella detection in blood: comparison of the BacT/Alert standard aerobic bottle, BacT/Alert FAN aerobic bottle and BacT/Alert enhanced FAN aerobic bottle.

Endocarditis is an inflammation of the endocardium, the membrane lining the chambers of the heart and covering the cusps of the heart valves. Infective.

Table 1. Diagnostic methods for systemic fungal infection (n=70)

Chapter 9 Preventing Infection Associated with Intravascular Therapy

Central venous catheter (CVC)-blood stream infection (BSI) rates.

Assessment of the BacT/Alert blood culture system: rapid bacteremia diagnosis with loading throughout the 24 h Jonas Bengtsson, Martin Wahl, Peter Larsson

Presentation transcript:

Catheter-related blood stream infections

Objectives 1.Catheter-related blood stream infections - Present scenario 2.Methods of diagnosing CRBSI 3.Importance of Differential Time to Positivity method by automated system 4.Interpret DTP result to diagnose CRBSI XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER 25-Nov-15

Catheter-related blood stream infections(CRBSI) Bacteremia/ fungemia in a patient with an intravascular catheter, associated with clinical manifestations of infections (i.e., fever, chills, and/or hypotension), and no apparent source for the BSI except the catheter Local signs of inflammation – absent in 70% of cases of CRBSI XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER 25-Nov-15

Diagnosis XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER 25-Nov-15

XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER (for bacteria) ≥25 CFU/ml (for fungal) 25-Nov-15

XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER (Maki method) (Endoluminal flush culture) 25-Nov-15

Procedure – Differential time to positivity XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER 25-Nov-15

Requirements: Automated blood culture system- BACTEC 9120 Bactec blood culture bottles (Paediatric and/or adult – Aerobic) XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER 25-Nov-15

Procedure 1 Two sets of blood collected simultaneously from the catheter hub of the CVC and from a peripheral site ml blood drawn aseptically 3 Inoculated into Bactec blood culture bottles which will be incubated in an automatic blood culture detection system 4 The time required for the bottles to become positive is recorded(TTP) 5 The differential time to positivity (DTP) is calculated XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER 25-Nov-15

XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER Collection time Positive signal Entry in BACTEC Arrival at Lab Identification and AST report Transport timeTime to positivity(TTP) Time to reporting TTP of catheter sample(TTP C ) TTP of peripheral sample(TTP P ) 25-Nov-15

XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER Peripheral Central CRBSI Negative ≥2 hours Same organism Positive Negative Different organisms Calculate DTP(TTP P -TTP C ) No Yes ????? Interpretation Excluded DTP cannot be measured Possible Contamination Local colonization 25-Nov-15

THANK YOU 25-Nov-15 XIII National Workshop on Diagnostic Methods in Clinical Microbiology, JIPMER