1680 1930 1960 1515. Optics Focus Dr. Marvin Minsky Confocal microscope: 1957.

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Presentation transcript:

Optics

Focus

Dr. Marvin Minsky Confocal microscope: 1957

LASER L ight A mplification by the S timulated E mission of R adiation Scanning Confocal Microscope Laser

Dichroic Mirror

Conventional / Confocal

The capability of an optical system to distinguish between two objects closely adjacent Resolution

The capability of an optical system to distinguish between two objects closely adjacent Resolution

The capability of an optical system to distinguish between two objects closely adjacent Resolution

Oversampling Resolution

Deconvolution

Conventional / Confocal / Biphoton

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Frequency Intensity Image treatment

Colocalization

Battista et al. Endocrinology 2009; 150: 4180 FIG 2. Immunofluorescence detection of seladin-1 in rat adult fasciculata cells Colocalization Seladin-1Golgi Merge + Mask

Demandolx et al. J of Microscopy 1997, 185: 21 FIG 1. Double immunofluorescence labelling of fibroblast transfectants monitored by confocal microscopy. Ia k  MHC class II molecules are represented in green and the Ii chain is represented in red. Colocalization