Denaturation temperatuare gradient- polymerase chain reaction: by-product? Ji Youn Lee Cell and microbial engineering laboratory Seoul National University.

Slides:

Advertisements

Similar presentations

The Polymerase Chain Reaction (PCR)

Advertisements

Genetic Engineering (and other cool molecular biology techniques)

C rime S cene I nvestigation. Who Ate The Cheese? Oh no! Someone ate the Queen ’ s prize Limburger cheese! Saliva samples were taken from the teeth marks.

Kurtis Cahill James Badal.  Introduction  Model a Maze as a Markov Chain  Assumptions  First Approach and Example  Second Approach and Example 

I-5-1 Basic Principles and Components of PCR NSYSU CHUNG-LUNG CHO.

PCR Overview Lifted from various powerpoint presentations on the internet.

General Genetics. PCR 1.Introduce the students to the preparation of the PCR reaction. PCR 2.Examine the PCR products on agarose gel electrophoresis.

Learning Objectives Draw or model the first three cycles of PCR, including the correct directionality (5’- and 3’-ends) of the primers and single-stranded.

DNA Replication DNA mRNA protein transcription translation replication Before each cell division the DNA must be replicated so each daughter cell can get.

PCR Primer Design Guidelines

Observation Hypothesis Experimental Design (including Methods) Results Inference Camp Wildness 2004 Ward Lab Research Project.

IN THE NAME OF GOD. PCR Primer Design Lecturer: Dr. Farkhondeh Poursina.

PCR optimization. Primers – design must be good but influenced by template sequence Quality of template DNA/impurities Components of PCR may need to be.

Polymerase Chain Reaction

Analysis of Hot Spring Microbial Mat

PCR By Staci Cutting and Mitch Gavazzi. What is PCR? PCR is sometimes called Molecular photocopying the polymerase chain reaction is a fast and inexpensive.

Polymerase Chain Reaction (PCR)

EDVOKIT#300: Blue/White Cloning of a DNA Fragment

Tools of Bioinformatics

Polymerase Chain Reaction. PCR Repetitive amplification of a piece or region of DNA Numerous uses –Straightforward amplification & cloning of DNA –RT-PCR.

What do these terms mean to you? You have 5 min to discuss possible meanings and examples with your group! DNA sequencing DNA profiling/fingerprinting.

Polymerase Chain Reaction (PCR) What is PCR?: Use of DNA polymerase to selectively amplify a segment of DNA from a much larger sample. Xeroxing DNA, start.

PCR = DNA ? LB 145 January 27, Does PCR happen inside your body? (How do you raise your temp to 95°C?) PCR was invented in the last 30 years. It.

DNA Technology Chapter 11. Genetic Technology- Terms to Know Genetic engineering- Genetic engineering- Recombinant DNA- DNA made from 2 or more organisms.

©2001 Timothy G. Standish Romans 5:17 17For if by one man’s offence death reigned by one; much more they which receive abundance of grace and of the gift.

It is a technique used to produce large quantities of replicated DNA.

Polymerase Chain Reaction (PCR)

INTRODUCTION. INTRODUCTION Introduction In the past, amplifying (replication) of DNA was done in bacteria and took weeks. In 1971, paper in the.

Polymerase Chain Reaction (PCR)

The polymerase chain reaction

Cell and Microbial Engineering Laboratory Solving TSP on Lab-on-a-Chip 1 Jiyoun Lee.

The polymerase chain reaction

Polymerase Chain Reaction A process used to artificially multiply a chosen piece of genetic material. May also be known as DNA amplification. One strand.

By: Cody Alveraz Ted Dobbert Morgan Pettit

2/5/15 Objective: How does DNA replication occur? Do Now: S1f0.

1 Naoto Takahashi, Atsushi Kameda, Masahito Yamamoto, and Azuma Ohuchi Hokkaido University, JST Aqueous Computing with DNA Hairpin-based RAM Summarized.

FOOTHILL HIGH SCHOOL SCIENCE DEPARTMENT Chapter 13 Genetic Engineering Section 13-2 Manipulating DNA.

Semiconservative DNA replication Each strand of DNA acts as a template for synthesis of a new strand Daughter DNA contains one parental and one newly synthesized.

Polymerase Chain Reaction: A Markov Process Approach Mikhail V. Velikanov et al. J. theor. Biol Summarized by 임희웅

Introduction to PCR Polymerase Chain Reaction

Polymerase Chain Reaction (PCR)

The first step to model DTG-PCR Ji Youn Lee Cell and microbial engineering laboratory Seoul National University.

Lab 22 Goals and Objectives: EDVOKIT#300: Blue/White Cloning of a DNA Fragment Calculate transformation efficiencies Compare control efficiency to cloned.

PCR Polymerase Chain Reaction PCR Polymerase Chain Reaction Marie Černá, Markéta Čimburová, Marianna Romžová.

Components Of A Typical PCR Reaction Mix PCR Reaction Buffer (usually supplied at 10X) 100mM Tris-HCl - Essentially a pH Buffer. Maintains appropriate.

The Search for a Jumping Gene: Module based on a kit from Bio-Rad Laboratories, Inc. Adapted by Dan Murray from a presentation by: Stan Hitomi - Monte.

PCR 의 kinetics 에 대한 좀더 많은 생각 … 2003 년 12 월 16 일 이지연.

I. PCR- Polymerase Chain Reaction A. A method to amplify a specific piece of DNA. DNA polymerase adds complementary strand DNA heated to separate strands.

1 Summarized by Ji Youn Lee. 2 Model Development.

Introduction to PCR Polymerase Chain Reaction

Polymerase Chain Reaction

Topics to be covered Basics of PCR

EDVOKIT#300: Blue/White Cloning of a DNA Fragment

PCR Polymerase Chain Reaction

POLYMERASE CHAIN REACTION TECHNIQUES

Molecular Cloning: Polymerase Chain Reaction

COURSE OF MICROBIOLOGY

Polymerase Chain Reaction

Polymerase Chain Reaction

Chapter 13.2 Manipulating DNA.

Crime Scene Investigation

Polymerase Chain Reaction

Polymerase Chain Reaction

Introduction to Bioinformatics II

Bioinformatics Lecture By: Ms AQSAD RASHDA

Phenylthiocarbamide (PTC)

Introduction to Polymerase Chain Reaction (PCR)

DNA Extraction -DNA exists in the nucleus of a eukaryotic cell; this means there are two membranes that separate the DNA from the environment -to get the.

Dr. Israa ayoub alwan Lec -12-

Polymerase Chain Reaction (PCR) & DNA SEQUENCING

Presentation transcript:

Denaturation temperatuare gradient- polymerase chain reaction: by-product? Ji Youn Lee Cell and microbial engineering laboratory Seoul National University

Let ’ s talk about DTG-PCR A newly invented tool by modifying the typical PCR for solving traveling salesman problem More amplification of DNA strands with lower melting temperature Is it True? Does it operate Well? Is it Worth?

It ’ s time to study DTG-PCR Experimental part Modeling and simulation part Make use of the published ones : Competitive PCR Addition of the ssDNA distribution profile with temperature

Let ’ s think about it!

Experimental considerations DNA strands with different Tms –Same length, different Tm –or Different length, different Tm –or Different length, same Tm … etc. Experiments –Mix the strands (two or more) –Perform DTG-PCR in various conditions –Analysis: TGGE, DGGE or graduate PCR

Theoretical considerations Studies up to date –Competitive PCR –Quantitative PCR –Internal standards and products –Anal Biochem (1993) Quantitative PCR: Theoretical considerations with practical implications Replication efficiency E (product and standard)

–NAR (1996) A mathematical model and a computerized simulation of PCR using complex templates Probability calculation –PNAS (1996) Efficiency of DNA replication in the PCR –NAR (1997) A coalescent approach to the PCR –NAR (1998) Modeling and analysis of competitive RT–PCR

–JTB (1997) Enzymological considerations for a theoretical description of the quantitative competitive PCR Michaelis-Menten equation (enzyme kinetics) –JTB (1997) Theoretical description of the PCR –JTB (1999) Polymerase chain reaction: Markove process approach –Bioinformatics (2001) Virtual PCR