NYKRCP004 RT- PCR report 2009. 09. 29 1. PCR Condition Temp.TimeCyclesFinal Reaction vol. 45 ℃ 30min1 50ul 95 ℃ 15min 1 95 ℃ 30sec 35 54 ℃ 30sec 72 ℃ 40sec.

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NYKRCP004 RT- PCR report PCR Condition Temp.TimeCyclesFinal Reaction vol. 45 ℃ 30min1 50ul 95 ℃ 15min 1 95 ℃ 30sec ℃ 30sec 72 ℃ 40sec 72 ℃ 10 min 1 4℃4℃ Storage 1 2. Primer set NAMEGENESize (bp) Pr7-F ORF7 FULL 662 Pr7-R NA-Pr5-F NA ORF5 FULL 716 NA-Pr5-R EU-Pr5-F EU ORF5 FULL 755 EU-Pr5-R NSP2-F NSP2 PARTIAL N:768 H: 678 NSP2-R

NYKRCP004 RT- PCR report #sample260/280260/230conc.(ng/ul) 1I I I I I I I / I / I / Sample list

3. The Result of PCR NYKRCP004 RT- PCR report 1 kb #GenePrimer setSize(bp) 1 ORF7 FULLPr7 F/R662 2NA ORF5 FULLNA-Pr5 F/R716 3NSP2 PARTIALNSP2 F/R bp 1kb #sample 1I I I I I I I /165 8I /167 9I /169 NA-Pr5 Pr7 NSP2

NYKRCP004 RT- PCR report RNA gel loading lanesampleconc.(ng/ul) Loading 양 1I ul 2I ul 3I ul 4I ul 5I ul 6I ul C C->Control RNA 1.4ug