PrimerSequence (5' to 3')Comments Hpt-FwCGCGACGTCTGTCGAGAAGTTT RT-PCR analysis Hpt-RvATCGGACGATTGCGTCGCATCGA RT-PCR analysis AOX1a-FwGATGTTTGTCTACTGCCGAGGATTT.

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a b 10A.zp85WT 10A.zp85S83μ 10A.vec 10A.z 10A.vec 10Az 10Az.p85WT

Supplementary Fig. 1.

Presentation transcript:

PrimerSequence (5' to 3')Comments Hpt-FwCGCGACGTCTGTCGAGAAGTTT RT-PCR analysis Hpt-RvATCGGACGATTGCGTCGCATCGA RT-PCR analysis AOX1a-FwGATGTTTGTCTACTGCCGAGGATTT RT-PCR analysis AOX1a-RvATGTAGTATATATAACTCAGCTGCC RT-PCR analysis AOX1b-FwTCATCATTCATCAACGGGCGATGC RT-PCR analysis AOX1b-RvTGTGCACGGGTCAGCCAACGGCCA RT-PCR analysis AOX1c-FwCTGAAGAAATCTTACGGCGG RT-PCR analysis AOX1c-RvCCAAACAGATAACAGGACGC RT-PCR analysis SpeI-AOX1A-NdeI-FwACTAGTATGAGCTCCCGGATGGCCG Plasmid construction for overexpression SpeI-AOX1A-NdeI-RvCATATGGTGATATCCGATCGGCGCAGG Plasmid construction for overexpression Actin1-FwATGCTCTCCCCCATGCTATC RT-PCR analysis Actin1-RvTCTTCCTTGCTCATCCTGTC RT-PCR analysis Supplementary Table 1. Primers used in this study. Table S1

Fig. S1 (a) (b) (c) (e) (f) SOD activity (units mg -1 protein h -1 ) a a a b b b b (d) GR activity (unit mg -1 protein) Time (h) Hyg 0 μ M Hyg 25 a b c c c c c μ M APX activity (unit mg -1 protein) Time (h) Hyg 0 Hyg 25 a b b b b b b μ M μ M Time (h) Hyg 0 Hyg 25 SOD activity (unit mg -1 protein h -1 ) a b cd c μ M μ M APX activity (units mg -1 protein) Hyg conc. ( a a b b b b b μ M ) Hyg conc. ( GR activity (unit mg -1 protein) Hyg conc. ( a a b b b b b μ M ) μ

(a) AOX1a Control Hyg (hr) Actin1 AOX1b AOX1c (b) AOX Control Hyg (hr) 34 kD 32 kD CBB Fig. S2