Inflammation Due To RA.

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Inflammation Due To RA.

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Presentation transcript:

Inflammation Due To RA

Normal Joint Bone

Normal Joint Bone Synovium

Normal Joint Bone Synovium Cartilage

Rheumatoid Arthritis Bone Destruction Synovial Inflammation

Osteoclasts

T helper Cells??

Th17 Cells

In Rheumatoid Arthritis, Do Th17 Cells play a major role in osteoclast differentiation in vivo?

Can osteoclast differentiation be induced in vitro by Th17 via RANK/RANKL mechanism?

Kim et al (2012)

RANKL

Kim et al (2012) RANKL

Kim et al (2012) RANKL Vitamin D

Kim et al (2012) RANKL Vitamin D 3 FOLD

Quantitative PCR After Reaction with RANKL

Quantitative PCR After Reaction with RANKL RT-PCR / Sybr Green TRAP RNA GAPDH reference gene

Since TRAP protein is only produced in osteoclasts, it can be used to measure differentiation.

Kim et al

% of GAPDH

Kim et al % of GAPDH Reduced Osteoclast Differentiation

Kim et al NO RANKL Reduced RANK expression

Kim et al Reduced RANK expression Reduction

Proposed Experiment T helper Cells

Proposed Experiment T helper Cells Vitamin D

Can osteoclast differentiation be induced in vitro by Th17 via RANK/RANKL mechanism? -Th17 in synovium -Capable of inducing differentiation via RANKL/Rank -Kim et al showed that Vitamin D inhibits via RANK

In Rheumatoid Arthritis, Do Th17 Cells play a major role in osteoclast differentiation in vivo? Use T cell specific RANKL knockout mice.