QUIZ Lab 2 Ag-Ab Reaction 10 points, 10 minutes Assist.Prof.Suwin Wongwajana,M.D.,M.Sc. Dept.of Microbiology, Fac of Medicine, KKU.

Write your name,student code,lab gr.Date on the top of the paper 8 items, 10 marks, 10 min.

1.Patient serum was diluted in serial 4- folded manner. After adding the Ag and let the reaction occurred. The precipitate was found until tube No.3. The antibody titer= ? (If dilution in tube No.1 = 1:4 ) a.1:4 b.1:16 c.1:24 d.1:64 e.1: 256

2. About single radial immunodiffusion (SRID), Which sentence is correct? a.It is an agglutination technique. b.It uses electricity in the test. c.It is sensitive than ELISA. d.It is a quantitative technique. e.All of the above.

3. The steps in immunoelectrophoresis (IEP) test included: a. let Ag react with Ab b. puncture the gel, add Ag into the well c. run the electrophoresis d. add Ab into the trough Which sequence is correct? a. abcdb. bcdac. cdba d. dabce. No correct answer

4. The results of immunodiffusion was shown, Which sentence is correct? a.Ag1and Ag2 are non-identity b.Ag1and Ag2 are partial identity c.Ag1and Ag3 are partial identity d.Ag2 and Ag3 are identity e.No correct answer Ab Ag3 Ag2 Ag1

5. About blood grouping test, If you have blood gr.O, Which sentence is correct? a.RBC have Ag A and Ag B. b.In serum have anti-A, No anti-B c.In serum have anti-B, No anti-A. d.RBC have no Ag A and no Ag B. e.No correct answer.

6. About ELISA technique, Which sentence is incorrect? 6. About ELISA technique, Which sentence is incorrect? a.Use enzyme as the labelling agent. b.Can detect both Ag or Ab. c.Must add the substrate then observing the color changes. d.The intensity of the color changed is direct correlated with the concentration of the unknown. e.Fluorescent microscope was required in order to assess the result.

7. Which sentence is incorrect? 7. Which sentence is incorrect? a.Gr O can give the RBC to the Gr.B b.Gr O can give the RBC to the Gr.AB c.Gr A can give the RBC to the Gr.AB d.The lowest percentage of Thai Bl.Gr.is AB e.The highest percentage of Thai Bl.Gr.is B

1 ml ml ml 1:4 1: ml Dilution Final Dilution

Answer No. 1-7, 1 mark each No. 8, 0.5 mark each Total 10 marks Total 10 marks

= 4 X 4 X 4 1.Patient serum was diluted in serial 4- folded manner. After adding the Ag and let the reaction occurred. The precipitate was found until tube No.3. The antibody titer= ? (If dilution in tube No.1 = 1:4 ) a.1:4 b.1:16 c.1:24 d.1:64 e.1: 256

a.It is an agglutination technique. b.It uses electricity in the test. c.It is sensitive than ELISA. d.It is a quantitative technique. e.All of the above. 2. About single radial immunodiffusion (SRID), Which sentence is correct?

3. The steps in immunoelectrophoresis (IEP) test included: a. let Ag react with Ab b. puncture the gel, add Ag into the well c. run the electrophoresis d. add Ab into the trough Which sequence is correct? a. abcdb. bcdac. cdba d. dabce. No correct answer

Ab Ag3 Ag2 Ag1 4. The results of immunodiffusion was shown, Which sentence is correct? a.Ag1and Ag2 are non-identity b.Ag1and Ag2 are partial identity c.Ag1and Ag3 are partial identity d.Ag2 and Ag3 are identity e.No correct answer

5. About blood grouping test, If you have blood gr.O, Which sentence is correct? a.RBC have Ag A and Ag B. b.In serum have anti-A, No anti-B c.In serum have anti-B, No anti-A. d.RBC have no Ag A and no Ag B. e.No correct answer.

6. About ELISA technique, Which sentence is incorrect? 6. About ELISA technique, Which sentence is incorrect? a.Use enzyme as the labelling agent. b.Can detect both Ag or Ab. c.Must add the substrate then observing the color changes. d.The intensity of the color changed is direct correlated with the concentration of the unknown. e.Fluorescent microscope was required in order to assess the result.

= O 7. Which sentence is incorrect? 7. Which sentence is incorrect? a.Gr O can give the RBC to the Gr.B b.Gr O can give the RBC to the Gr.AB c.Gr A can give the RBC to the Gr.AB d.The lowest percentage of Thai Bl.Gr.is AB e.The highest percentage of Thai Bl.Gr.is B

1 ml 9 1:1 00 1:8, :1 0 1:80, ml ml 1:4 1: 80 1:1 00 1:8,00 0,000 1 ml 3 Dilution Final Dilution8.

Write the-name of the checker -Total score Send them back to teacher No. 1-7, 1 mark each No. 8, 0.5 mark each Total 10 marks Total 10 marks

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