Atmospheric Pressure Ionization - Time of flight mass spectrometry (API-TOF/MS) API-TOF/MS is the integration of two instrumental techniques, API and TOF/MS.

Slides:

Advertisements

Similar presentations

FC-MS from Teledyne Isco CombiFlash ® a Name You Can Rely On.

Advertisements

Mass Spectrometry Mass spectrometry involves ionizing a compound, separating the ions that result on the basis of mass to charge ratio (m/z) and obtaining.

Atomic Mass Spectrometry Yongsik Lee Introduction ► Atomic mass spectrometry  Versatile and widely used tool  All elements can be determined ►

Module 2: Analytical methods and separation techniques.

Mass spectroscopy. In a typical MS procedure:  1- a sample is loaded onto the MS instrument, and undergoes vaporization.  2- the components of the sample.

Mass Spectroscopy Skyline IB Chemistry HL Mass Spectroscopy.

DRUG TESTING 17 February Vocab Review What is the difference between a presumptive test and a confirmatory test? Presumptive tests have high false.

Atomic Emission Spectroscopy

427 PHC.  Atomic emission spectroscopy (AES) is based upon emission of electromagnetic radiation by atoms.

Mass Spectrometry Inlet system Ion Source Mass Analyzer Ion Detector.

Mass Spectrometry The substance being analyzed (solid or liquid) is injected into the mass spectrometer and vaporized at elevated temperature and reduced.

Mass Spectroscopy Mass Spectrometry ä Most useful tool for molecular structure determination if you can get it into gas phase ä Molecular weight of.

Lecture 8. GC/MS.

Chem. 133 – 4/28 Lecture. Announcements Lab Report 2.3 due Today Pass back graded materials (lab reports 2.2, Q5, and AP3.1) Today’s Lecture Mass Spectrometry.

Instrumental Chemistry Chapter 11 Atomic Mass Spectrometry.

LC-MS Based Metabolomics. Analysing the METABOLOME 1.Metabolite Extraction 2.Metabolite detection (with or without separation) 3.Data analysis.

Proteomics The proteome is larger than the genome due to alternative splicing and protein modification. As we have said before we need to know All protein-protein.

Atomic Mass Spectrometry

Molecular Mass Spectroscopy Chem Introduction In Mass Spectroscopy (MS), atomic and molecular weights are generally expressed in terms of atomic.

Atomic Mass Spectrometry

LC-MS Lecture 7.

Mass Spectroscopy Quantitative Chemical Analysis Harris, 6th Edition

Atomic Mass & Molar Mass

INSTRUMENTAL ANALYSIS CHEM 4811

LC/MS WORKSHOP IOWA STATE UNIVERSITY Kamel Harrata  Instrument Description  Data Acquisition  Data Processing.

Instant Notes Analytical Chemistry

Mass Spectrometer Mass spectrometere is a very powerful method to analyse the structure of organic compounds.

Mass spectrometric methods Assistant of the pharmaceutical chemistry department Burmas Nataliya Ivanivna

Mass spectrometry is an analytical tool that makes use of the mass to charge ratio of particles to determine the molecular formula of a compound. In the.

Analytical Chemistry Option A Part 1: Mass Spectrometry & H-NMR.

Atomic Emission Spectroscopy

Chemical Analysis. Analytical Techniques When chemical evidence is collected at a crime scene, it must be run through an instrument. These instruments.

Mass spectrometry Ions are analyzed on the basis of their m/z Chlorine has 2 isotopes, 35 Cl and 37 Cl, in the approximate ratio of 3 :1. Electrons are.

Molecular mass spectrometry Chapter 20 The study of “molecular ions” M + e -  M e -

Based on ionization of gas phase molecule followed by analysis of the masses of the ions produced. The Mass Spectrum: Graph of ion intensity versus mass-to-charge.

Chemistry Topic: Atomic theory Subtopic : Mass Spectrometer.

7-1 Atomic Mass Spectroscopy General Features Mass Spectrometers §Sources Atomic mass spectroscopy determines elements by mass §Mass to charge ratio §Lower.

Mass spectrometry (Test) Mass spectrometry (MS) is an analytical technique that measures masses of particles and for determining the elemental composition.

Mass Spectrometry Instrumentation: Ionization methods and analyzers.

Drs. Wei Tian & Yanhui Chen Sep-Dec Main Content General Introduction of Mass spectrometry (MS) Time of Flight Mass Spectrum （ TOF-MS ） (Key point)

Methods of Chemical Analysis. Selecting an Analytical Technique  Organic vs. Inorganic materials  Organic:  Inorganic:  Quantitative vs. Qualitative.

Dr. Kristoffer Rem Labing-isa Massachusetts Institute of Technology Dean, College of Science.

Mass Analyzers : Time-of-flight Mass Spectrometry CU- Boulder CHEM 5181 Mass Spectrometry & Chromatography Joel Kimmel Fall 2007.

F LORIDA I NTERNATIONAL U NIVERSITY Advanced Mass Spectrometry Piero R. Gardinali/Yong Cai/ Bruce McCord Revised on August 23, 2009.

Secondary Ion Mass Spectrometry A look at SIMS and Surface Analysis.

LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY

Chromatography and Instrumentation. Chromatography Separate Analyze Identify Purify Quantify Components Mixture Chromatography is used by scientists to:

Mass Spectrometry Relative atomic masses and the mass of individual isotopes can be determined using a mass spectrometer. The principle behind mass spectrometry.

Chem. 133 – 4/26 Lecture. Announcements Return graded quiz and additional problem Lab – Lab report deadlines (2:4 – Thursday) Today’s Lecture – Mass Spectrometry.

Chapter 29 Mass Spectrometry. 29 A Principles of mass spectrometry In the mass spectrometer, analyte molecules are converted to ions by applying energy.

Presented by – ANAMIKA BANERJEE (RESEARCH SCHOLAR)

Objective  To develop methods for analysis of compounds in organic aerosol particles Why is this important?  Environmental impact  Alternative fuels.

What is Mass Spectrometry? Mass spectrometry could be considered as an analytical technique that involves the study in the gas phase of ionized molecules.

Lab Tools of Forensic Scientists. Detection Presumptive tests ◦ Give idea as to what probably is in a sample/what definitely is not in a sample  Ex:

Unit 3: Identifying elements and molecules Unit Objectives: Explain how mass spectrometry is used to identify different substances. Deduce the identify.

Atomic Structure Topic The mass spectrometer Describe and explain the operation of a mass spectrometer Describe how the mass spectrometer.

Mass Spectrometry u Chapter 12 Chapter 12.

Acknowledgements Slides and animations were made by Dr. Jon Karty Mass Spectrometry Facility Indiana University, Bloomington.

Mass Spectrometry Obaid M. Shaikh.

Organic Chemistry Lesson 18 Mass spectrometry.

Starter Complete the quiz and hand in..

Mass Spectroscopy. Mass Spectroscopy Mass Spectrometry Most useful tool for molecular structure determination if you can get it into gas phase Molecular.

Instrumental Chemistry

Instrumental Chemistry

Mass Spectrometry CHEM HONORS.

Molecular Mass Spectroscopy

Mass Spectrometry THE MAIN USE OF MS IN ORG CHEM IS:

Compound is first vaporized and converted into ions, which are then separated and detected. Electron Impact (EI) Mass Spectrometry 1.

M.Prasad Naidu MSc Medical Biochemistry, Ph.D,.

Mass spectrometry (MS) is an analytical technique that can be used to determine the mass, elemental composition or chemical structure of molecules. Mass.

Presentation transcript:

Atmospheric Pressure Ionization - Time of flight mass spectrometry (API-TOF/MS) API-TOF/MS is the integration of two instrumental techniques, API and TOF/MS. Both are relatively simple and are based on fundamental ideas about charged particles. But fundamentals first: What is API What is TOF/MS Integration of API and TOF/MS into one Why API-TOF/MS

API Chemical ionization in the air at atmospheric pressure.  -rays produced by tungsten wire. Inelastic collisions between carrier gas Ionization of He carrier gas –He + + A ==> He + A +

TOF/MS Analyte is usually ionized by a focused laser. Vaporized and ionized particles are forced down a drift tube by means of an applied potential to the tube. Separation occurs due to differing m/z. Lightest ones first, heaviest last (1 to 30  s). Ion transducer is in essence a PMT. Internal TOF/MS pressure is between to torr.

Integration of API to TOF/MS Two main hurdles: –Differing pressures between API and TOF/MS –TOF/MS requires a pulse for time determination. Pressure gradient: –Differentially pumped interfaces. Pulse production: “ion packet” –Plate Deflector method

Why API-TOF/MS Increased resolution Low detection capabilities, 100 femtomolar range. –Multi-hit method. –Single-hit method. API promotes “soft ionization” Non-volatile substances can be ionized more easily Full mass spectrum can be taken on every injection. High mass compounds can be determined. Easy to use and relatively inexpensive.