Transcriptomics Sequencing. over view The transcriptome is the set of all RNA molecules, including mRNA, rRNA, tRNA, and other non coding RNA produced.

Slides:



Advertisements
Similar presentations
Transcriptomics Breakout. Topics Discussed Transcriptomics Applications and Challenges For Each Systems Biology Project –Host and Pathogen Bacteria Viruses.
Advertisements

Peter Tsai Bioinformatics Institute, University of Auckland
LONG NON-CODING RNA PHANG LAB TALK MAY 03, Transcriptome The collection of all transcripts (RNA) presents in a given cell ~5 % codes for proteins.
Transcriptomics Jim Noonan GENE 760.
© 2006 W.W. Norton & Company, Inc. DISCOVER BIOLOGY 3/e
Gene Expression Overview
Investigating the Importance of non-coding transcripts.
RNA-seq Analysis in Galaxy
Chapter 15 Noncoding RNAs. You Must Know The role of noncoding RNAs in control of cellular functions.
E. coli RNA Polymerase Sequences of E. coli Promoters.
mRNA-Seq: methods and applications
Identification of obesity-associated intergenic long noncoding RNAs
Before we start: Align sequence reads to the reference genome
Control of Gene Expression Eukaryotes. Eukaryotic Gene Expression Some genes are expressed in all cells all the time. These so-called housekeeping genes.
Transcription Transcription is the synthesis of mRNA from a section of DNA. Transcription of a gene starts from a region of DNA known as the promoter.
Epigenome 1. 2 Background: GWAS Genome-Wide Association Studies 3.
Introns and Exons DNA is interrupted by short sequences that are not in the final mRNA Called introns Exons = RNA kept in the final sequence.
Transcriptome analysis With a reference – Challenging due to size and complexity of datasets – Many tools available, driven by biomedical research – GATK.
AP Biology From Gene to Protein How Genes Work AP Biology What do genes code for? proteinscellsbodies How does DNA code for cells & bodies?  how are.
Experimental validation. Integration of transcriptome and genome sequencing uncovers functional variation in human populations Tuuli Lappalainen et al.
Next Generation Sequencing and its data analysis challenges Background Alignment and Assembly Applications Genome Epigenome Transcriptome.
RNA and Protein Synthesis
GENOM REGULATION BY LONG NONCODING RNA SUPERVISOR: DR.FARAJOLLAHI PRESENTED BY: BAHAREH SADAT RASOULI.
Expression of the Genome The transcriptome. Decoding the Genetic Information  Information encoded in nucleotide sequences contained in discrete units.
Small RNAs and their regulatory roles. Presented by: Chirag Nepal.
Stefan Aigner Christian Carson Rusty Gage Gene Yeo Crick-Jacobs Center Salk Institute Analysis of Small RNAs in Stem Cell Differentiation.
Marco Magistri , Journal Club. A non-coding RNA (ncRNA) is any RNA molecule that is not translated into a protein “Structural genes encode proteins.
Centra Dogma Primer. Structure of DNA and RNA Nucleic acids made of nucleotides G, A, T/U, C Ribose vs. deoxyribose Template-dependent synthesis Double.
AP Biology From Gene to Protein How Genes Work.
The generalized transcription of the genome Víctor Gámez Visairas Genomics Course 2014/15.
8.6 Gene Expression and Regulation TEKS 5C, 6C, 6D, 6E KEY CONCEPT Gene expression is carefully regulated in both prokaryotic and eukaryotic cells.
Predicting protein degradation rates Karen Page. The central dogma DNA RNA protein Transcription Translation The expression of genetic information stored.
Xiaole Shirley Liu STAT115, STAT215, BIO298, BIST520
Prokaryotic cells turn genes on and off by controlling transcription.
Eukaryotic Gene Structure. 2 Terminology Genome – entire genetic material of an individual Transcriptome – set of transcribed sequences Proteome – set.
Post-transcriptional regulation
PLANT BIOTECHNOLOGY & GENETIC ENGINEERING (3 CREDIT HOURS) LECTURE 13 ANALYSIS OF THE TRANSCRIPTOME.
Starter What do you know about DNA and gene expression?
CFE Higher Biology DNA and the Genome Transcription.
A new player in the innate immunity game? Scientists have demonstrated for the first time that a certain class of RNA (known as long non-protein-coding.
1 From Bi 150 Lecture 0 October 4, 2012 An introduction to molecular biology... but you will learn the cell biology in this course.
Alessandro Raganelli and Varun Rao.  Prokaryotes and eukaryotes alter gene expression in response to their changing environment  In multicellular eukaryotes,
Unit 1: DNA and the Genome Structure and function of RNA.
Mestrado Integrado em Medicina Biologia Celular e Molecular II
Click to continue How do a few genes build a diversity of body parts? There’s more in the genetic toolkit than just genes! Click your forward cursor to.
RNA-Seq with the Tuxedo Suite Monica Britton, Ph.D. Sr. Bioinformatics Analyst September 2015 Workshop.
3rd Internal RECESS workshop Caroline C. Friedel
The Transcriptional Landscape of the Mammalian Genome
Eukaryotic Gene Structure
RNA-Seq analysis in R (Bioconductor)
DNA Transcription.
Exam #1 W 9/26 at 7-8:30pm in UTC 2.102A Review T 9/25 at 5pm in WRW 102 and in class 9/26.
Prokaryotic cells turn genes on and off by controlling transcription.
Gene Regulation Ability of an organisms to control which genes are present in response to the environment.
Prokaryotic cells turn genes on and off by controlling transcription.
Transcription -The main purpose of transcription is to create RNA from DNA because RNA leaves the nucleus to carry out its functions but DNA does not -A.
From Gene to Protein How Genes Work.
DNA Transcription.
Prokaryotic cells turn genes on and off by controlling transcription.
mRNA Degradation and Translation Control
RNA sequencing (RNA-Seq) and its application in ovarian cancer
Continues Probability Distributions and Estimation
Prokaryotic cells turn genes on and off by controlling transcription.
The Structure of the Genome
Unit 1: 1.5 Structure of the Genome
Prokaryotic cells turn genes on and off by controlling transcription.
Prokaryotic cells turn genes on and off by controlling transcription.
Sequence Analysis - RNA-Seq 1
Prokaryotic cells turn genes on and off by controlling transcription.
Presentation transcript:

Transcriptomics Sequencing

over view The transcriptome is the set of all RNA molecules, including mRNA, rRNA, tRNA, and other non coding RNA produced in one or a population of cells. RNA Next-generation sequencing methods have revolutionized exploration of the transcriptome.

The transcriptomics sequencing is to sequence the mature mRNA and ncRNA which transcribed by affinity purified RNA polymerase II with next generation sequencing.transcriptomics The Target of Sequencing

Advances in the sequencing workflow, from sample preparation through data analysis, enable rapid profiling and deep investigation of the transcriptome. Advantage

The Trancsriptomics Sequencing of CD Genomics The transcriptomics sequencing service of CD Genomics include: RNA-Seq (Transcriptome):RNA-Seq is a powerful tool for studying the effect of the transcriptome on information for biological, medical, clinical and pharmaceutical research, drug discovery and development.RNA-Seq RNA-Seq (Quantification):RNA-Seq (Quantification) based on Next Generation Sequencing (NGS) is used for transcriptome quantification and characterizing gene expression profiling.Quantification Small RNA Sequencing:Small RNA has been to important regulators in recent years and has significant functions in many cellular processes including development, cell differentiation and apoptosis, which has been implicated in many diseases.Small RNA Sequencing lncRNA: Long non-coding RNA (lncRNA) is a major hotspot in functional genomics research that considered as non-protein-coding transcripts longer than 200 nucleotides in length.lncRNA

Service include: Annotate coding SNPs Discover transcript isoforms Identify regulatory RNAs Characterize splice junctions Determine the relative abundance of transcripts

Work flow(example of lncRNA):