AP Lab Skills Guide

AP Lab Skills Guide

+ AP Lab Skills Guide Mix contents Begin Timing Tube 1 (substrate) Tube 2 (enzyme) Tube 3 (tubes 1 + 2) Begin Timing H2O2 Guaiacol Distilled H2O Peroxidase Distilled water

AP Lab Skills Guide Each minute for 5 min compare the color of tube 3 to the standard Time (min) Color 1 2 3 4… Tube 3 (tubes 1 + 2) Begin Timing Graph the data…

AP Lab Skills Guide Time (min) Color 1 2 3 4 5 6 7 8 10 Each minute for 5 min compare the color of tube 3 to the standard You are determining how long to let the reaction go to get a value of “5” as your baseline for comparisons in the next step…you will see…we will run all future reactions for 5 minutes and look at color and compare to enzyme in distilled water….

AP Lab Skills Guide

AP Lab Skills Guide Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Tube 6 Peroxidase H2O2 pH 12 buffer Peroxidase H2O2 pH 2 buffer Peroxidase H2O2 pH 4 buffer Peroxidase H2O2 pH 6 buffer Peroxidase H2O2 pH 8 buffer Peroxidase H2O2 pH 10 buffer Cover with parafilm, gently mix and measure color at 0 minutes and color change at time determined in part 1 (5 minutes)...

AP Lab Skills Guide pH Color 2 1 4 6 5 8 3 10 12 Of course, we should combine the class data, take averages, SD, SE and graph with error bars…

AP Lab Skills Guide

AP Lab Skills Guide

AP Lab Skills Guide

AP Lab Skills Guide

AP Lab Skills Guide