EFFECTS OF CHRONIC ALCOHOL ON BEHAVIOR AND ALPHA-2 ADRENOCEPTORS IN TWO RAT STRAINS B. Getachew*, S. R. Hauser, J. R. Das, C. Ramlochansingh, B. Bhatti,

Slides:



Advertisements
Similar presentations
Lifespan of C. elegans Separation of transgenic animals from nontransgenic animals Isolation of first-day adult worms Placement of first day adult worms.
Advertisements

Biological Explanations of Aggression
ALCOHOL WITHDRAWAL RESULTS IN DEPRESSIVE-LIKE BEHAVIOR IN RATS Bruk Getachew, Sheketha R. Hauser, Robert E. Taylor, Yousef Tizabi Howard University, College.
Do Ultrasonic Vocalizations During Intermittent Swim Stress Forecast Resilience in a Subsequent Forced Swim Test?  There was no difference in behaviour.
Ethological Evaluation of the “Spontaneous” and stress-related Anxiety-like Behavior During Ethanol Withdrawal: Long-Term Impact of Alcohol Dependence.
CORTICOSTERONE MODULATES ETHANOL WITHDRAWAL- MEDIATED HIPPOCAMPAL DAMAGE: RELATION TO ELEVATED CA 2+ LEVELS PJ Mulholland 1, RL Self 1, BR Harris 2, HJ.
Investigating a Novel Neutral Antagonist, 6Beta-Naltrexol, in Alleviating Cocaine Withdrawal Symptoms Timothy DeYoung, Michelle Mueller, and Dr. Boyette-Davis.
Risk for psychiatric diseases following cannabis abuse in adolescence :an experimental study Daniela Parolaro Univ of Insubria.
The Effect of a High Fat Diet and Enriched Environment in Rat Performance in the 8-Arm Radial Maze Task and the Forced Swimming Test J. H. Calderon, V.
Short and long-term effect of growth hormone on basal and catecholamine induced lipolysis in pigs The Message Short-term treatment with growth hormone.
 Left Panel. The two highest nicotine doses (0.8 and 2.0 mg/kg) produced a CTA on several sessions and the test session in the WKY rats. (* = 0.8 mg/kg.
Examination of Age-Related Cholinergic Activity during Nicotine Exposure and Withdrawal Luis M Carcoba, M.D, Ph.D. University of Texas at El Paso Department.
March 6th, 2009ASENT meeting / Juha RouruOrion Group Orion Pharma 1 ORM for negative and cognitive symptoms of schizophrenia, depression and Alzheimer’s.
Investigating the behavioral responses to developmental nicotine exposure in zebrafish Amanda Slade Mike Simonich Tanguay Lab September 24, 2009.
Lynda Sharrett-Field Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.
Prof. Flavia Franconi University of Sassari. Pharmacogenetics chronopharlacology Pharmacogenetics chronopharlacology Drugs Pharmacokinetics Modulators.
Synergistic Action of 17-  estradiol and Bisphenol A in a Pituitary Cell Line Sarah Korb and Winnifred Bryant, Ph. D. Department of Biology University.
Crocins, the active constituents of Crocus Sativus L., antagonized psychotomimetic effects produced by the NMDA receptor antagonist ketamine in rats G.
W HEEL R UNNING A LTERS S EROTONIN (5-HT) T RANSPORTER, 5-HT 1A, 5-HT 1B, AND A LPHA 1 B -A DRENERGIC R ECEPTOR M RNA IN THE R AT R APHE N UCLEI Greenwood.
Using the FST to Correlate Depressive-Like Behavior and Estrogen Levels in Three Strains of Mice Kunwal Nasrullah Columbia University, College of Physicians.
Taurine Supplementation Improves the Utilization of Sulfur-Containing Amino Acids in Consecutive Alcohol Administration Rats Hui-Ting Yang 1, Ching-Chien.
Left and Right Panels. Mice treated with prazosin were less active than their vehicle-paired counterparts (left panel). Time course analysis shows paired.
Dendritic complexity is reduced in neurons of nucleus accumbens after social isolation Ue-Cheung Ho 1,2, Yu-Chun Wang 1,2, Chun-Chieh Liao 2 and Li-Jen.
DESIPRAMINE BLOCKS THE DEPRESSIVE EFFECTS OF ALCOHOL IN FEMALE WKY RATS Sheketha R. Hauser 1*, Bruk Getachew 2, Robert E. Taylor 2, Yousef Tizabi 2 1 Department.
CONTEXT FEAR CONDITIONING INHIBITS ACTIVE DEFENSIVE BEHAVIOR ELICITED BY ELECTRICAL STIMULATION OF DORSAL PERIAQUEDUCTAL GRAY: RELATIONSHIP BETWEEN ANXIETY.
Does Scheduled Intermittent Access to a Highly Palatable Carbohydrate-Rich Food Trigger Binge Eating in Laboratory Rats? Robynn Mackechnie, Kishan Govind,
Determining the Effect of Triclosan on the Growth of Cancer Cells Lydia Alf and Winnifred Bryant Ph. D. Department of Biology University of Wisconsin,
Expression of egr 1 (zif 268 ) mRNA in select fear-related brain regions following exposure to a predator Jeffrey B. Rosen, Robert E. Adamec, Barbara L.
Role of muscarinic M1 receptors in inhibitory avoidance and contextual fear conditioning Juliana Carlota Kramer Soares, Raquel Vecchio Fornari, Maria Gabriela.
Withdrawal-induced Inhibition of Phagocytosis in Morphine Tolerant Macrophages may be Due to an Increase in cAMP.
The Hypothalamopituitary-adrenal axis and alcohol preference Matthew J. O’Callaghan, Adam P. Croft, Catherine Jacquot, Hillary J. Little Presented by Muharema.
Role for Hypocretin in Mediating Stress-Induced Reinstatement of Cocaine-Seeking Behavior Investigating the effects of Hypocretin-1+2 (Hcrt-1 / Hcrt-2)‏
Chronic treatment with cannabinoid receptor agonist, CP 55,940, alters GLT1 expression in adolescent rats Introduction Methods Results Selected References.
Damian G. Zuloaga ¹, John A. Morris ², Cynthia L. Jordan ¹, ², S. Marc Breedlove ¹, ² ¹Department of Psychology; ² Neuroscience Program Michigan State.
E ff ects of duloxetine in rats trained to discriminate between 2- and 22- hr food deprivation M.A. Vanden Avond, A.R. Johnson, K.A. Reed, B.R. Gomer, D.J.
The Effects of Vegetation, Nutrition, and Sex Ratio on the Reproductive Cycle of Fundulus heteroclitus in the Laboratory College of Arts and Sciences,
The goal of this communication is to show that (i) actomyosins with different calcium sensitivity interact with each other; (ii) the desensitized actomyosin.
Does daily limited access to a highly palatable sweet food induce binge eating in laboratory rats? Katie Isbell, Robynn Mackechnie, Pres Garrett, and G.R.
Introduction Zebrafish (Danio rerio) have become an increasingly popular animal model for the study of anxiety. A variety of studies have already begun.
Raj Solanki ENDOCANNABINOID & STRESS SYSTEM INTERACTIONS.
Identification of Brain Regions Activated with Arousal- Induced Clock Resetting in Mice Jonathan E. Schenk, Andrew T. Schultz, and Robert G. Olson with.
Adenosine Protects Vascular Barrier Function in Hyperoxic Lung Injury Jonathan Davies 1, Harry Karmouty-Quintana 2, Thuy T. Le 2, Ning-Yuan Chen 2, Tingting.
Probiotic Bacteria Prevent Gut Permeability During Heat Stress Iryna Sorokulova, Ludmila Globa, Oleg Pustovyy, Vitaly Vodyanoy Department of Anatomy, Physiology.
Behavioral and Medical Trends in Sensitivity to the Effects of Caffeine Abstract Caffeine has unquestionably become the world’s most popular psychomotor.
Margaret Della Vecchia 2 and Anthony S. Rauhut 1,2, Department of Psychology 1 and Neuroscience Program 2, Dickinson College, Carlisle, PA References Discussion.
CB1R activation enhances hippocampal excitatory neurotransmission in female adolescent rats Christian G. Reich and Joseph Boscarino Psychology Program,
Timing of SCH23390 Administration Influences Extinction of Conditioned Hyperactivity in Mice Anthony S. Rauhut 1,2, Kristen Ratner2, Sandy Buck2, and Ee-Rah.
Modelling Madness Susan Totterdell.
Yan Li1, Connie Sánchez1, Maria Gulinello2
Morozova A.Y., Zubkov E.A., Chekhonin V.P.
BEHAVIORAL AND HORMONAL EFFECTS OF CHRONIC RESTRAINT STRESS IN ADOLESCENT AND ADULT RATS Hansen C, Virgolini MB, De Giovanni L, Miranda-Morales RS, Willie-Billie.
COMPARATIVE TOXICITY STUDY OF CHLOROQUINE AND HYDROXYCHLOROQUINE ON ADULT ALBINO RATS M.A. El Shishtawy,K. Haidar Hassan .Department of Forensic Medicine.
Discussion & Conclusion
Species Comparison of the Binding Properties for Three D2/3-DAR
Volume 48, Issue 6, Pages (December 2005)
Randolph-Macon Woman’s College
Overeating Induced by Injection of the Glutamate Receptor Antagonist, CNQX, within the Nucleus Accumbens of Satiated Rats Project Leader: Jenny McCallister.
Volume 60, Issue 2, Pages (October 2008)
Corticotropin-Releasing Factor in the BNST
Liang Yang, Yong Qi, Yunlei Yang  Cell Reports 
Volume 71, Issue 4, Pages (August 2011)
“Say hello to my little neuropeptide”
A Sordid Tale of Antagonism
Wallis, JD Helen Wills Neuroscience Institute UC, Berkeley
Volume 42, Issue 5, Pages (June 2004)
NPS and Food intake.
Kanyan Xu, Xiangzhong Zheng, Amita Sehgal  Cell Metabolism 
Dopamine-Dependent Interactions between Limbic and Prefrontal Cortical Plasticity in the Nucleus Accumbens: Disruption by Cocaine Sensitization  Yukiori.
Volume 66, Issue 6, Pages (June 2010)
The effect of roscovitine on Ca2+-dependent glutamate release from synaptosomes prepared from rat brains in response to membrane depolarization with 1.
Presentation transcript:

EFFECTS OF CHRONIC ALCOHOL ON BEHAVIOR AND ALPHA-2 ADRENOCEPTORS IN TWO RAT STRAINS B. Getachew*, S. R. Hauser, J. R. Das, C. Ramlochansingh, B. Bhatti, R. E. Taylor, Y. Tizabi Department of Pharmacology, College of Medicine, Howard University, Washington, DC INTRODUCTION Alpha-2 adrenoceptors (alpha-2 ARs) are involved in a variety of physiological and behavioral functions including depression and drug dependence. Studies suggest that increased alpha-2 AR density or sensitivity results in insufficient neuronal release of NE which may lead to depression. Moreover, a variety of antidepressant drugs and other treatments of depression are associated with decreases in the density and sensitivity of both central and peripheral alpha-2 ARs. Alpha-2 ARs are also thought to be involved in alcohol’s action. However, very little information on a direct interaction between alcohol and these receptors is available. The aims of this study were to: 1. determine whether inherent differences in alpha-2 AR densities exist between a putative animal model of depression, namely Wistar-Kyoto (WKY) rats and their control Wistar (WIS) rats, and 2. determine the effects of chronic alcohol administration on behavioral indices of depression and on the density of alpha-2 ARs in discrete brain areas of both rat strains. WKY rats, derived from WIS rats are considered a suitable animal model of depression as they exhibit exaggerated immobility in the forced swim test (FST) compared to WIS rats. Our hypotheses were that depressive-like behavior in WKY rats is associated with higher alpha-2 AR density compared to WIS. Furthermore, alcohol administration would induce depressive-like behavior in WIS rats and exacerbates it in WKY rats and these behavioral effects are associated with increases in alpha-2 AR binding in both strains. Figure 4. Effect of 10 day daily alcohol vapor exposure on alpha-2 AR levels in different brain regions of WKY rats. Values are mean ± SEM. N=7-8. ***P< 0.001, **P 0.01 compared to WKY control rats. SUMMARY OF RESULTS Chronic alcohol treatment resulted in a depressive-like behavior in WIS rats and exacerbated this characteristic in WKY rats. Alpha-2 ARs were higher in several brain regions (cortical areas and midbrain) of WKY rats compared to WIS. Alcohol treatment resulted in a down-regulation of these receptors in the cortical and midbrain areas of WKY rats, whereas WIS rats were not affected in any region. ACKNOWLEDGEMENTS Supported by NIAAA (P20 AA014643), AA and NIH/NIGMS (2 SO6 GM ). METHODS Animals Age matched adult female WKY and WIS rats (Harlan) were kept in a temperature-controlled room (24-26 o C) on a 12:12 hour reversed light/dark cycle (lights on at 19:00). The animals had ad libitum access to food and water, except during experiments. Alcohol Vapor Exposure and Behavioral Testing WKY and WIS rats were exposed 4 hrs daily to alcohol via inhalation chambers (La Jolla Alcohol Research Inc. San Diego, CA) for 10 days. The blood alcohol concentration in both strains was maintained at approximately 150 mg% during the exposure period. On day 11 automated analysis of locomotor activity (LCA) in an open field was performed for 10 min prior to the Forced Swim Test (FST). FST, a modification of the method of Porsolt et al.(1977), measures immobility of animals in an inescapable cylinder of water. The total amount of time the animal demonstrates this behavior reflects the animal's state of behavioral despair. The animals were placed in the water cylinders for 5 minutes, videotaped and their swimming and immobility were scored at every 5 second interval according to Detke et al. (1995). Tissue Preparation and Receptor Binding Two hours after the behavioral test, the animals were sacrificed for measurement of alpha-2 AR densities in discrete brain regions. Tissues were homogenized in ice-cold buffer (50 mM Tris-HCl, 3 mM MgCl2, and 1 mM EGTA, pH 7.4) and centrifuged at 48,000-x g at 4°C for 30 minutes. The pellet was re-suspended and store at -80°C till receptor binding. Alpha-2 AR levels were measured using 2.5 nM [ 3 H]RX in the presence of unlabeled norepinephrine. CONCLUDING STATEMENT These results do not support a direct role for central alpha-2 ARs in the inherent depressive-like characteristics of WKY rats or its exacerbation by alcohol. Similarly, no correlation between alcohol-induced depressive-like behavior and alpha-2 AR in WIS rats was noted. Thus, it may be suggested that basal alpha-2 AR densities and their response to alcohol are genetically determined and appear to be independent of the depressive-like behavior. Figure 2. Baseline alpha-2 AR levels in different brain regions of WKY and Wistar rats. Values are mean ± SEM. ***P< 0.001, **P 0.01 compared to Wistar rats. N=7-8. Figures 1A and B. Effect of 10 day daily alcohol vapor exposure on locomotor activity and forced swim test immobility counts of WKY and Wistar rats. Values are mean ± SEM. N=7-8. ***P< Fig. 1A LCA Fig. 1B FST RESULTS Figure 3. Effect of 10 day daily alcohol vapor exposure on alpha-2 AR levels in different brain regions of Wistar rats. Values are mean ± SEM. N=7-8.