Principles of Biostatistics ANOVA. DietWeight Gain (grams) Standard910 8 Junk Food10 13 12 Organic91012910 Table shows weight gains for mice on 3 diets.

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Presentation transcript:

Principles of Biostatistics ANOVA

DietWeight Gain (grams) Standard910 8 Junk Food Organic Table shows weight gains for mice on 3 diets. Test the following hypothesis at the α = 0.05 sig level.

DietWeight Gain (grams) Standard910 8 Junk Food Organic Table shows weight gains for mice on 3 diets. Test the following hypothesis at the α = 0.05 sig level. ANOVA (Analysis of Variance) Assumptions: Samples are independent (within and among groups) Population variances are equal Populations are normally distributed

Table shows weight gains for mice on 3 diets. Test the following hypothesis at the α = 0.05 sig level. DietWeight Gain (grams)Mean Standard Junk Food Organic

Table shows weight gains for mice on 3 diets. Test the following hypothesis at the α = 0.05 sig level. DietWeight Gain (grams)Mean Standard Junk Food Organic DietSquare Deviations (within groups) Standard(9-9.4) 2 (10-9.4) 2 (8-9.4) 2 Junk Food( ) 2 ( ) 2 ( ) 2 Organic(9-10) 2 (10-10) 2 (12-10) 2 (9-10) 2 (10-10) 2 DietSquare Deviations (within groups) Standard Junk Food Organic10410

Table shows weight gains for mice on 3 diets. Test the following hypothesis at the α = 0.05 sig level. DietWeight Gain (grams)Mean Standard Junk Food Organic DietSquare Deviations (within groups) Standard Junk Food Organic10410

Table shows weight gains for mice on 3 diets. Test the following hypothesis at the α = 0.05 sig level. DietWeight Gain (grams)Mean Standard Junk Food Organic DietSquare Deviations (between groups) Standard( ) 2 Junk Food( ) 2 Organic( ) 2 DietSquare Deviations (between groups) Standard0.87 Junk Food1.60 Organic0.11

Table shows weight gains for mice on 3 diets. Test the following hypothesis at the α = 0.05 sig level. DietWeight Gain (grams)Mean Standard Junk Food Organic DietSquare Deviations (between groups) Standard0.87 Junk Food1.60 Organic0.11

Table shows weight gains for mice on 3 diets. Test the following hypothesis at the α = 0.05 sig level. DietWeight Gai (grams)Mean Standard Junk Food Organic

Table shows weight gains for mice on 3 diets. Test the following hypothesis at the α = 0.05 sig level. DietWeight Gai (grams)Mean Standard Junk Food Organic

Calculating the p-value

Post-hoc Tests DietWeight Gain (grams) Standard910 8 Junk Food Organic

Post-hoc Tests DietWeight Gain (grams) Standard910 8 Junk Food Organic

Post-hoc Tests DietWeight Gain (grams) Standard910 8 Junk Food Organic Assumptions Samples are independent Population variances are equal Populations are normally distributed Not paired samples

Estimating the Pooled Variance DietWeight Gain (grams)St Dev Standard Junk Food Organic

Back to the Post-hoc Tests DietWeight Gain (grams)Mean Standard Junk Food Organic

Post-hoc Confidence Interval DietWeight Gain (grams)Mean Standard Junk Food Organic

Post-hoc Summary DietWeight Gain (grams)Mean Standard Junk Food Organic t-statisticDecision -2.81Reject H Retain H Retain H 0 Confidence Interval (-3.91, -0.49) (-2.31, 1.11) (-0.11, 3.31)

SPSS Output

Bonferroni’s Correction Example A study has 3 groups. 3 comparisons must be made. 1 to 2, 1 to 3, 2 to 3 If the pairwise error rate is 5%, approximate the overall error rate Answer: 15%

Bonferroni’s Correction Example A study has 4 groups. 6 comparisons must be made. 1 to 2, 1 to 3, 1 to 4, 2 to 3, 2 to 4, 3 to 4 If the pairwise error rate is 5%, approximate the overall error rate Answer: 30%

Bonferroni’s Correction Example A study has 3 groups. 3 comparisons must be made. 1 to 2, 1 to 3, 2 to 3 If the overall error rate is 5%, approximate the pairwise error rate Answer:

Bonferroni’s Correction Example A study has 4 groups. 6 comparisons must be made. 1 to 2, 1 to 3, 1 to 4, 2 to 3, 2 to 4, 3 to 4 If the overall error rate is 5%, approximate the pairwise error rate Answer:

Formula for # of Comparisons

Post-hoc Confidence Intervals with Bonferroni’s Correction

DietWeight Gain (grams)Mean Standard Junk Food Organic

SPSS Output