Laser Flow Cytometry Forward Scatter indicates size Forward Scatter.

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Presentation transcript:

Laser Flow Cytometry

Forward Scatter indicates size Forward Scatter

Side Scatter indicates complexity Side Scatter

Fluorescent wavelength detector FITC labeled cell! Fluorescence Measurements

PE labeled cell! Fluorescence Measurements Fluorescent wavelength detector

Fluorescence Detection FL-1: FITC, CFSE, ALEXA FL-2: PE, PI FL-3: Texas Red, Red-670 FL-4: APC, Topro

This demonstrates gating by size and complexity based on forward scatter and side scatter, respectively. Size and Complexity Separation

Negative Control This sample is a negative control. It has no conjugated stains and represents the overall background. No Stain

CD4:FITC No Stain Single Staining This sample has a single stain of CD4 labelled with FITC stain, giving a green color to those cells expressing CD4.

No Stain CD8:PE Single Staining This sample has a single stain of CD8 labelled with PE stain, giving an orange color to those cells expressing CD8.

CD4:FITC CD8:PE Double Staining This sample has a double stain of CD4:FITC and CD8:PE, giving a green and orange color to those cells expressing both CD4 and CD8.