The NucleoCounter® NC-100™ Fast, precise and objective counting and viability determination of mammalian cells

NucleoCounter® NC-100™ key advantages and selling points Easy operation – no preparation 30 sec. analysis time Calibration free No cleaning Maintenance and service free Portable/compact Safe sample handling and disposal High precision & reproducibility

NucleoCounter® NC-100™ - Mammalian cells NucleoCounter is optimized to count mammalian cells cultured in TC-flasks, bioreactors, micro carriers etc.

NucleoCounter® NC-100™ Concept: 5 components NucleoCounter® NC-100 ™ NucleoCassette Lysis Buffer Stabilizing Buffer NucleoView Software

Easy Operation LyseLoad Count

Propidium Iodide 1 dye molecule per 4-5 bp Genome between 2.5 x 10 9 – 4.0 x 10 9 bp. DNA base pair (bp): A: Adenine T: Thymine G: Guanine C: Cytosine C 27 H 34 N I - Mr 668.4

Limitations of present methods of cell counting

Cell Counting, Whole Nuclei Using Fluorescent DNA Dye PI Hard to discriminate cells from debris by microscopy Easy to count nuclei by fluorescence microscopy

Integrated Fluorescence Microscope Lenses: magnification <1X. Excitation filter: sorts out green light. Emission filter: sorts out long wave length including red light. fixture

Integrated Fluorescence Microscope

The NucleoCassette™

Nuclei of Mammalian cells Different in cell size: Diameter between  m. Nuclei are almost uniform in size:  m. Genome between 2.5x10 9 – 4.0x10 9 bp. Advantage: No calibration to cell type (size) using the NucleoCounter NC-100.

Reagent A-100 and B Lysis reagent A-100: Disrupts the plasma membranes of the mammalian cells, rendering the nuclei susceptible to staining with PI Disaggregation of cell clusters Stabilizing reagent B: Raises the pH value, allowing PI to stain the DNA of the cells more efficiently

Viability Determination

NucleoCounter® NC-100™ comparison with conventional cell counting methods

NucleoView  Software

Technical Specifications

Validation IQ/OQ

Typical NC-100 customers

NucleoCounter® NC-100™ key advantages and selling points Easy operation – no preparation 30 sec. analysis time Calibration free No cleaning Maintenance and service free Portable/compact Safe sample handling and disposal High precision & reproducibility