Week 10 Review: Old Business Documents P15080 Katelyn Busse Collin Burkhart Michelle Garafolo Robert Repetti Morgan Stoessel Sarah Tran

Customer Requirements ●Revision 1 ●Most Important Customer Requirements ○Ability to control shear stress ○Sterility ○Temperature ○% Relative Humidity ○%CO2 ●Approved by customer on 9/8/2014 ●Customer Requirements EDGECustomer Requirements EDGE ●Customer Requirements Google SheetsCustomer Requirements Google Sheets

Engineering Requirements ● Revision 3 o ER6-Cells can be viewed using Fluorescence o ER9-Assembly Time o ER15-Surface Area of Adhesion Surface o ER18-Size of Chamber o ER22-Size of Channel o Linked to FMEA and Test Plan ●Engineering Requirements EDGEEngineering Requirements EDGE ●Engineering Requirements Google SheetsEngineering Requirements Google Sheets ● Important ERs o Shear Stress o Size of Channel o Sterility o Ease of Assembly ● Approved by Customer on 10/26/14

Functional Decomposition ● Revision 2 o Further developed as the design progressed o Connected back to relevant CRs and ERs ●Functional Decomposition EDGEFunctional Decomposition EDGE ●Functional Decomposition Google DriveFunctional Decomposition Google Drive

Morph Chart 1 ●Revision 2 ●Functions/Solutions to original Functional Decomp ●Ideal choice colored Green ●Back-up choice (if applicable) colored Yellow ● Updated to include links to Customer and Engineering Requirements. ●Morph Chart EDGEMorph Chart EDGE ●Morph Chart Google SheetsMorph Chart Google Sheets

● Pugh EDGE Pugh EDGE ●Pugh Google SheetsPugh Google Sheets Concept Selection (PUGH) ● Revision 2 ● Datum selected revised to link to Customer and Engineering Requirements ● Note the total advantages of the “Incubator and Pump Design One”

FMEA ● Added failure modes based on preliminary test plan and engineering requirements o Channel Dimensions o Cell growth in tubing o Cells don’t adhere o Pressure in chamber ●FMEA EdgeFMEA Edge ●FMEA Google DriveFMEA Google Drive

System Architecture ● Improved System Diagram o Labelled arrows o Minor adjustments - filter, microscope slide ● Specified Interface Operation o Listed system interfaces o Identified and managed integration issues ●Edge - System Architecture DiagramEdge - System Architecture Diagram ●Edge - InterfacesEdge - Interfaces ●System Architecture Diagram - Google SlidesSystem Architecture Diagram - Google Slides ●Interfaces - Google DocsInterfaces - Google Docs

Pump Cell Chamber Media Reservoir Electricity Flow Rate Cells Microscopes Alternate Reservoir Media Media Color HeatCO 2 Water Pan ArchitectureColor Structure Energy Information Materials Incubator Filter System Architecture Media Light CO 2 (moves while still attached) Pump Setting (swap) (pumped into incubator) Heating Element Power Cord Fresh Media (seeded) Microscope Slide (rests in a groove within) Systems Architecture Diagram on Edge

Interfaces ● Growth Chamber o Base/O-ring o O-ring/Lid o Base/Lid o Base/Slides o Base/Inlet Tube o Base/Outlet Tube Clamping Points Lid Inlet Opening O-ring Recess Slide Recesses Channel Base

Interfaces ● Media Reservoir o Reservoir/Lid o Reservoir/Media o Lid/Filter o Lid/Inlet Tube o Lid/Outlet Tube ● Pump o Pump/Pump Head o Pump Head/Tubing ● Tubing o Tubing/Media ● Slides o Slide/Protein o Protein/Cells

Interfaces ● User o Shear Input o Cell Input o Media Input o Temperature Output o Humidity Output o Media Quality Output ● Energy o Electricity Input o Heat Input

Risk Management Largests Risks as of 10/23/14 Fluid Reservoir design Shear Stress Proving Shear Stress Controlling Pump Duke University Intellectual Property Meeting Deadlines Risks EDGE

Bill of Materials: Estimated Budget ● Revision 2 o Added adapters and stopcocks o Specified certain products (brands) o More refined estimates o Total Estimated Cost: $ ● BOM Edge BOM Edge ● BOM Google Sheets BOM Google Sheets