Group: C1 Ko – Locaylocay In vitro prebiotic effect of Zingiber officinale (ginger) on Lactobacillus Group: C1 Ko – Locaylocay

Introduction Ginger Zingiber officinale underground rhizome - herbal medicinal part Main components: sesquiterpenoids, nonvolatile phenylpropanoids (gingerol & zingerone), diarylheptanoids Gingerol – antioxidant, antipyretic, antimicrobial, antiemetic

Introduction Prebiotic Non-digestible food ingredient – stimulate the growth or activity of one or more bacteria in the colon Dietary fibers – polysaccharides, lignins & oligosaccharides

Introduction Lactobacillus Gram-positive, facultative anaerobe or strictly aerobic bacteria Lactic acid bacteria group Normal flora of vagina, GIT & GUT

Significance of the Study Competitive exclusion principle Protection against gastric disturbances Preventive measure for colon cancer

Research Question Does ginger have prebiotic effect on Lactobacillus in vitro?

Objectives General Objective: To determine the prebiotic effect of ginger on Lactobacillus in vitro.

Objectives Specific Objectives: 1. To determine whether ginger flesh extract can up-regulate growth of Lactobacillus in in vitro conditions. 2. To determine which ginger extract concentration is the most effective in enhancing the growth of Lactobacillus. 3. To determine the degree and significance of growth up-regulation ginger flesh extracts exert on Lactobacillus in vitro.

Methodology

Aqueous Ginger Flesh Extract Preparation

Aqueous Ginger Flesh Extract Preparation

Standardization of Broth Colony and Turbidity Testing

Total Bacterial Count

Statistical Analysis ANOVA

Group: C1 Ko – Locaylocay In vitro prebiotic effects of Zingiber officinale (ginger) on Lactobacillus Group: C1 Ko – Locaylocay