Microscopy Supervised by : Dr. Sherifa Prepared by : Reem Aldossari Lama Al abdi.

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Presentation transcript:

Microscopy Supervised by : Dr. Sherifa Prepared by : Reem Aldossari Lama Al abdi

Genera!y, It is an instrument used to examine and monitor objects we can’t see with the naked eye. It has many applications. Microscope

There are different classes and types of microscopes depending on the magnification power, their applications, and how they are operated: Simple Microscopes Inverted microscope. Light (compound) microscope. Fluorescent Microscope Types of microscopes

-Simple Microscopes A magnifying glass, an ordinary double convex lens having a short focal length, is a simple microscope. -A simple microscope has only one lens. Simple Microscopes

The compound microscope is made of 3 systems: The mounting and movement System. The magnification System. The iumination System. Compound Microscope

The mounting and movement system The body tube: carries the ocular lenses The arm: Supports and connects the upper part of the microscope The coarse focusing knob: for stage movement The fine focusing knob: for image sharpness The nose piece: Carries the objective lenses and move them accordingly above the stage The stage: Horizontal platform upon which the slide of interest rest The base: Supports the microscope

The ocular lenses: 5X, 10X, and 15X The objective lenses: Scanning lens: 3.5X or 4X or 5X Low power lens: 10X High power lens: 40X Oil immersion lens: 100X THE MAGNIFICATION SYSTEM How to calculate the magnification power? Magnification power = ocular lens xobjective lens. (e.g.) 10X x 40X = 400X hint: Don’t forget the unit

THE ILLUMINATION SYSTEM The illuminator: light source The iris diaphragm: controls the amount of light reaching the slide The condenser: collects and concentrate the light

 The light source and the condenser are placed on top of the stage while as the objective lenses are placed underneath pointing up.  Useful for observing living cells or organisms at the bottom of a large container. Inverted Microscope

Fluorescent Microscope The "fluorescence microscope" refers to any microscope that uses fluorescence to generate an image.

Histo Chemical stains Glycogen ( carmine stain )

Protein ( bromophenol blue )

Lipids ( Sudan black B)

Thank you for your attention