Production of Erythropoietin in Pichia Pastoris Eporis – Team 15 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps.

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Presentation transcript:

Production of Erythropoietin in Pichia Pastoris Eporis – Team 15 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

Team Eporis 1/10 Abby Leistra Stephen Tubergen Zion LeeNick Giles StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

Background: What is Erythropoietin? Erythropoietin (EPO) is a glycoprotein hormone Naturally made in kidneys Promotes red blood cell production Treats anemia caused by Chronic kidney disease Cancer chemotherapy HIV therapy $4 Billion US Market in 2013 glycam.org 2/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

Problem Chinese Hamster Ovary (CHO) Cells Slow-growing Many purification steps nikon2.magnet.fsu.edu Solution 2013.igem.org Pichia Pastoris Cells Fast-growing Easy purification of protein 3/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

EPO Structure 4/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps Nett, J. H.; Gomathinayagam, S.; et. al. J. Biotechnol. 2011, 157, Polyethylene Glycol (PEG) Large Negatively charged

Capacity Estimate How much? How big? Depends on: Market Volume Yeast Productivity Separation Recovery en.wikipedia.org Bottom Line: 169 g EPO/yr → 8500 L/yr 5/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

Process Model -PFD 6/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

Process Model -Bioreactor Key Considerations Oxygen Distribution Impeller Shear Force 7/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

Process Model - Filtration Ultrafiltration Microfiltration Houp, R. C. J. Validation Tech. 2009, /10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

Business Model Three different alternatives Start –up Contracting Leasing 9/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps stcaustralia.org

Future Steps Provide detail to the process model Calculating flow rates in and out of major process units Determining process subunits Evaluate alternatives Business model Process units Optimize selected process model 10/10 StructureReviewProcess ModelCapacityBusiness ModelFuture Steps

Questions?