Revision!Revision! L.O: to summarise biochemical tests to apply knowledge to questions.

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Presentation transcript:

Revision!Revision! L.O: to summarise biochemical tests to apply knowledge to questions

Biochemical Tests are used to indicate the presence / absence of biological molecules… r/chemicals/biologicalcompoundsact.shtmlhttp:// r/chemicals/biologicalcompoundsact.shtml

Summary Table Biological Molecule Biochemical test Positive Result

Summary Table Biological Molecule Biochemical test Positive Result Protein Lipid Reducing Sugar Non Reducing Sugar

Summary Table Biological Molecule Biochemical testPositive Result ProteinBiuretPurple Lipid Reducing Sugar Non Reducing Sugar

Summary Table Biological Molecule Biochemical testPositive Result ProteinBiuretPurple LipidEmulsion Test (ethanol + H 2 O) White precipitate Reducing Sugar Non Reducing Sugar

Summary Table Biological Molecule Biochemical testPositive Result ProteinBiuretPurple LipidEmulsion Test (ethanol + H 2 O) White precipitate Reducing Sugar Benedicts + HEAT Brick red Non Reducing Sugar

Biological Molecule Biochemical testPositive Result ProteinBiuretPurple LipidEmulsion Test (ethanol + H 2 O) White precipitate Reducing Sugars (mono and most di) Benedicts + HEATBrick red Non Reducing Sugar (sucrose) 1. Hydrolyse into monomers by HCL + boiling. 2. Neutralise with alkali. 3. Retest with Benedicts + HEAT Brick red

Application of Knowledge

Question 1 a)A = Protein B = Lipid C = Reducing Sugar b)Hydrolyse with acid and then neutralise with alkali, re test with Benedicts c)Carbon, Hydrogen, Oxygen

Question 1 (again!) 1 a) C 12 H 22 O 11 (Because H2O is formed in the condensation reaction) 1 bi) Purple 1 bii) Because it’s a protein 1 c) Benedicts AND heat Brick Red

Application of Knowledge

Question 1 (again!!) 1 a) Nitrogen 1 b i) Benedicts AND heat Brick Red 1 b ii ) Substance A (glucose) 1 c i) Forming bonds, with the removal of water 1 c ii) C (protein) 1 d i) Substance D 1 d ii) Maximum amount of hydrogen atoms NO double bonds / all carbons attached to two hydrogens