Genetic Testing Jeopardy! Waterford Union High School

Rules Each team sends one person per turn. They cannot get help from their team First to “buzz” in gets 15 seconds to answer. If the first person to buzz cannot get the answer, the second team can buzz in. Once they buzz in, they too have 15 seconds. They also cannot get help. You either gain or lose points on each turn. If no team buzzes within 10 seconds, the question ends and the answer is given.

$100 $200 $300 $400 $500 Sanger Method PCR Electroph. DNA FP’s & RFA Southern Blotting ELISAThis & That

Sanger Method This is a “fake” nucleotide that is dyed and allows DNA to be read letter by letter by stopping the addition of bases at random points ddNTPs

Sanger Method This separates the DNA fragments from longest to shortest Electrophoresis Gel

Sanger Method These are the kind of cells we’d use to sequence DNA in most cases White blood cells

Sanger Method These are the portions of DNA that code for actual genes and have an Open Reading Frame Exons

Sanger Method This is what is used to replicate the DNA for the Sanger Method E. coli

PCR Electrophoresis These are the regions of DNA that have random repeats of short sequences of bases STRs

PCR Electrophoresis Based on the information in the table below, this suspect’s DNA was found at the scene of the crime Suspect 2

PCR Electrophoresis This is the enzyme taken from hot springs that is used in PCR to make copies of DNA in a heating/cooling environment like that of PCR Taq polymerase

PCR Electrophoresis DNA is denatured in PCR using this Heat

PCR Electrophoresis This is the term for the process of separating DNA fragments after they’ve been cut and amplified Electrophoresis

DNA Fingerprints & Restriction Fragment Analysis This is when variations in a person’s DNA are examined to determine what aspects are unique to an individual DNA Fingerprinting

DNA Fingerprints & Restriction Fragment Analysis This where patterns created by cut DNA in a gel are examined to determine the source of DNA, paternity, or diagnose a disease. Restriction Fragment Analysis

DNA Fingerprints & Restriction Fragment Analysis This kind of DNA is used to create the DNA Fingerprint because it contains the most variability and STRs Introns

DNA Fingerprints & Restriction Fragment Analysis Human DNA is ___% the same from person to person 99.9

DNA Fingerprints & Restriction Fragment Analysis This is necessary to see the DNA after it is run through a gel Dying

Southern Blotting This is the genetic test we’d use to diagnose a genetic disease Southern Blotting

To conduct Southern Blotting, we’d use this to transfer DNA from the gel. A membrane

Southern Blotting To conduct Southern Blotting, we’d use this to find a specific gene in question due to the fact that this has a complementary sequence to that DNA Probe

Southern Blotting A probe will usually do this if a gene is present Glow (bioluminescence)

Southern Blotting Southern Blotting does not work for this kind of disease Infectious

ELISA This is the kind of test we’d use for infectious disease. ELISA

ELISA can detect infectious disease by looking for this substance Proteins (or antibodies)

ELISA These are produced to identify substances in your body Antibodies

ELISA These are recognized by antibodies and are a sign of a disease in the body Antigens

ELISA During ELISA, if a disease is present, the blood sample will ______ Show a color

This & That This is the kind of DNA that codes for a protein Exon

This & That This is the kind of DNA that does not code for a protein Intron

This & That This is how we know if a stretch of DNA is an exon or an intron Open Reading Frame

This & That This is the term for an antibody generator Antigen

This & That This is the enzyme that cuts DNA Restriction enzyme