Introductory Protein Structure Warren Kaplan Peter Wills Bioinformatics Centre Garvan Institute of Medical Research.

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Presentation transcript:

Introductory Protein Structure Warren Kaplan Peter Wills Bioinformatics Centre Garvan Institute of Medical Research

2. A little about Deep View Available from: Runs on Windows, Mac, Linux/FreeBSD, Irix (SGI) Free for all, but no source code I don’t get paid to say nice things about Deep View

3. Introduction to Protein Structure

Primary Structure >1DN1:A SYNTAXIN BINDING PROTEIN 1 MAPIGLKAVVGEKIMHDVIKKVKKKGEWKVLVVDQLSMRMLSSCCKMTDIMTEGITIVED INKRREPLPSLEAVYLITPSEKSVHSLISDFKDPPTAKYRAAHVFFTDSCPDALFNELVK SRAAKVIKTLTEINIAFLPYESQVYSLDSADSFQSFYSPHKAQMKNPILERLAEQIATLC ATLKEYPAVRYRGEYKDNALLAQLIQDKLDAYKADDPTMGEGPDKARSQLLILDRGFDPS SPVLHELTFQAMSYDLLPIENDVYKYETSGIGEARVKEVLLDEDDDLWIALRHKHIAEVS QEVTRSLKDFSSSKRMNTGEKTTMRDLSQMLKKMPQYQKELSKYSTHLHLAEDCMKHYQG TVDKLCRVEQDLAMGTDAEGEKIKDPMRAIVPILLDANVSTYDKIRIILLYIFLKNGITE ENLNKLIQHAQIPPEDSEIITNMAHLGVPIVTDSTLRRRSKPERKERISEQTYQLSRWTP IIKDIMEDTIEDKLDTKHYPYISTRSSASFSTTAVSARYGHWHKNKAPGEYRSGPRLIIF ILGGVSLNEMRCAYEVTQANGKWEVLIGSTHILTPQKLLDTLKKLNKTDEEISS >1DN1:B SYNTAXIN 1A MKDRTQELRTAKDSDDDDDVTVTVDRDRFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSA ILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQH STLSRKFVEVMSEYNATQSDYRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFASG IIMDSSISKQALSEIETRHSEIIKLENSIRELHDMFMDMAMLVESQGEMIDRIEYNVEHA VDYVERAVSDTKKAVKYQSKARRKKIM

Peptide Backbone There’s more to Alanine than just an A 3 Letter code Each amino acid has a unique structure Uniqueness of the R group Cheat using DeepView

Secondary Structure Alpha Helices Beta Sheets/Strands Loops/ coils

Select: Secondary Structure H=helix; B=residue in isolated beta bridge; E=extended beta strand; G=310 helix; I=pi helix; T=hydrogen bonded turn; S=bend 1 MAPIGLKAVV GEKIMHDVIK KVKKKGEWKV LVVDQLSMRM LSSCCKMTDI HHHHH HHHHHHHTTG GGTTTT EE EEE HHHHHH HHHHTTHHHH 51 MTEGITIVED INKRREPLPS LEAVYLITPS EKSVHSLISD FKDPPTAKYR TTTTB EEEE TTS BTT SEEEEEE HHHHHHHHHT TTTGGG BS 101 AAHVFFTDSC PDALFNELVK SRAAKVIKTL TEINIAFLPY ESQVYSLDSA EEEEEESS HHHHHHHHH TGGGGTBSEE EE SB BTTEEE T 151 DSFQSFYSPH KAQMKNPILE RLAEQIATLC ATLKEYPAVR YRGEYKDNAL THHHHHH ST TTTSHHHHHH HHHHHHHHHH HHHT EE EETTTHHHHH 201 LAQLIQDKLD AYKADDPTMG EGPDKARSQL LILDRGFDPS SPVLHELTFQ HHHHHHHHHH HHHTTTTTTT STTGGG EE EEEEGGG TT TTTS BHH 251 AMSYDLLPIE NDVYKYETSG IGEARVKEVL LDEDDDLWIA LRHKHIAEVS HHHHHHS B TTEEEEEE S SSS EEEEEE STT HHHHH HTTTBHHHHT 301 QEVTRSLKDF SSSKRMNTGE KTTMRDLSQM LKKMPQYQKE LSKYSTHLHL HHHHHHHHHH HHHTT SS T TTT GGGHHH HHHHHHHHHH 351 AEDCMKHYQG TVDKLCRVEQ DLAMGTDAEG EKIKDPMRAI VPILLDANVS HHHHHHHHHT THHHHHHHHH HHHHTB SS B THHHH HHHHHTTTS 401 TYDKIRIILL YIFLKNGITE ENLNKLIQHA QIPPEDSEII TNMAHLGVPI HHHHHHHHHH HHHHTT B H HHHHHHHHHH T HHHHTHH HHGGGGT 451 VTDSTLRRRS KPERKERISE QTYQLSRWTP IIKDIMEDTI EDKLDTKHYP B GGG S TT HHHHHHHHHH HT S TTTEE 501 YISTRSSASF STTAVSARYG HWHKNKAPGE YRSGPRLIIF ILGGVSLNEM SS EEEEE EET EEHHHH 551 RCAYEVTQAN GKWEVLIGST HILTPQKLLD TLKKLNKTDE EISS HHHHHHHHHH SS EEEEEES SEE HHHHHH HHTTTTS SS

Domains Cn3D from the NCBI

Tertiary Structure The coming together of secondary structure elements

Quartenary Structure Molecules of proteins associate to form multimers Colour: Chain

Anatomy of a PDB file Header PDB code Remark Resolution Remark 350 Generating biological multimer SEQRES Sequence HELIX SHEET SSBOND CRYST1 ATOM TER HETATM CONNECT END

Bonds Covalent Disulfide Bridges Backbone Non-Covalent (Van der Waals Interactions) Hydrogen Bonds Salt Bridges

4. Analysis Tools

Manipulating the molecule Rotating In a plane Translating Zooming

Selecting and Displaying Control Panel: Select all, Chain, Secondary Structure, Individual

Colouring Control Panel:Colour Individual, ranges Colour entire structure e.g Ribbons

Measuring and labelling Menu Bar Select: Neighbours of selected amino acid

Ramachandran Plot Identify Manipulate N terminus C terminus