Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.

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Presentation transcript:

Biofilms

Dental plaque

Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants 4- Associated diseases

Tubing obstruction Equipamiento dental >30años

MS 2- M+M+

Biofilm formation on a voice implant

Contact lens

Catheter

P. Aeruginosa biofilm in lung

Structure

Biofilm formation process

Glass surface in the lab

MBFHBFLBFWT Citrate Glucose+Caa Genetic analysis of biofilm formation

Genetic characterization of biofilm formation - Requirement of movement - Adhesins -Signaling -Glycocalyx formation

Movement requirement -Flagelar-mediated mobility -Non-motile mutants in P. Fluorescens -Twitching -Mutants in type IV pili in P. aeruginosa -Suppression by cells “stickier” -ompR and adhesin Curli in E. coli

Adhesins -Type I pilus (fimH) in E. coli -Type IV (MSHA) pilus in V. cholerae -Curli adhesin

Signaling -Quorum sensing

QUORUM SENSING:DENSITY-DEPENDENT EXPRESSION CULTURE DENSITY AUTOINDUCER TRESHOLD

GRAM-NEGATIVE COMMUNICATION: THE LuxI/LuxR LANGUAGE LuxI LuxR Target gene

GRAM-NEGATIVE COMMUNICATION: THE LuxI/LuxR LANGUAGE -Light production in Vibrio fisheri -Virulence in Pseudomonas aeruginosa (LasI/R, RhlI/R) -Opine formation in Agrobacterium tumefaciens (TraR/I)

GRAM-positive COMMUNICATION: THE PHEROMONE LANGUAGE ABC D Target gene Pheromone gene H

HYBRID LANGUAGES: THE CASE OF Vibrio harvey LuxO Target gene LuxN LuxLMLuxS LuxQ

-Competence for DNA uptake in Bacillus subtilis and Streptoccocus pneumoniae (ComX/ComP/ComA) -Sporulation in Bacillus subtilis -Conjugation in Enteroccus fecalis -Virulence in Staphylococus areus GRAM-positive COMMUNICATION: THE PHEROMONE LANGUAGE

QS-mutants

Glycocalyx formation -Alginate production in P. aeruginosa - EPS in V. cholerae (vps)

fimA lasI vps flagella rhl alg fimH Curli

Natural process of biofilm formation / Control by a blocking analogue