MICROSCOPE DESIGN. beam expander and objective Why do we need a beam expander? What facts about the laser do we need to know? How much expansion do we.

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Presentation transcript:

MICROSCOPE DESIGN

beam expander and objective

Why do we need a beam expander? What facts about the laser do we need to know? How much expansion do we need? What is lens J for? K? L? What physical constraints are important to keep in mind? How do we focus?

(beam size on sample) (beam size at lens L) = (apparent focal length of objective) (focal length of lens L) note: apparent focal length of objective = 200 mm/(magnification)

LJKexpansion 100 mm25 mm75 mm3x 150 mm25 mm or 50 mm 100 mm or 200 mm ~4x 200 mm25 mm150 mm6x for a 40x objective

What if we want to use the 100x objective? Do we have to change everything or start over?

dichroic mirror, filter, and mirror

What is the dichroic mirror for? What is the filter for? Why is that mirror there? What does it do?

lens Q and the CCD

What facts about the CCD do you need to know? Why must lens Q be 200 mm?