Construction of a Genetic Toggle Switch in Escherichia coli

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Presentation transcript:

Construction of a Genetic Toggle Switch in Escherichia coli All diagrams except the for the detailed switch diagram taken from: Gardner, T.S., Cantor, C.R. and Collins, J.J. (2000)Construction of a Genetic Toggle Switch in Escherichia coli Nature 403 (6767) 339-343

Design U = concentration repressor 1 V = concentration repressor 2 α = effective rates of synthesis β and γ = cooperativity of repressors

Detail

Specifications In absence of inducer, only one promoter should be effectively active Addition of inducer (eg. IPTG or thermal pulse) should trigger switching to desired state

Product 3 Promoter systems: Low State: PLs1con (CI) (Stronger) PLtetO-1 (TetR) v u High State: Ptrc-2 (LacI)

Characteristics η = cooperativity of IPTG binding K = dissociation constant

Pros Cons . Is completely stable in both states Can be switched between states easily Small construct so products can be placed downstream Requires protein degradation so takes a LONG time to switch between states IPTG cannot be removed from environment after induction

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