Label-Free Protein Biosensor Based on Aptamer-Modified Carbon Nanotube Field-Effect Transistors Kenzo Maehashi, Taiji Katsura, Kagan Kerman, Yuzuru Takamura,

Slides:

Advertisements

Similar presentations

Tunable Surface Assembly of Gold Nanorods for Biosensor Applications

Advertisements

Uses of Cloned Genes sequencing reagents (eg, probes) protein production insufficient natural quantities modify/mutagenesis library screening Expression.

by Alexander Glavtchev

6.1 Transistor Operation 6.2 The Junction FET

Conclusions Future work Methods Background Introduction Lily Stanley, Juan Du, and Xuan Gao Department of Physics, Case Western Reserve University Nanowire.

Atomistic Simulation of Carbon Nanotube FETs Using Non-Equilibrium Green’s Function Formalism Jing Guo 1, Supriyo Datta 2, M P Anantram 3, and Mark Lundstrom.

Aptamers in the Real World Development and Applications NeoVentures Biotechnology Inc. Aptamers Applied1.

Carbon nanotube field effect transistors (CNT-FETs) have displayed exceptional electrical properties superior to the traditional MOSFET. Most of these.

Modulation of Conductance in a Carbon Nanotube Field Effect Transistor by Electrochemical Gating - A pplication to the detection of unique sequences of.

Non Specific Binding (NSB) in Antigen-Antibody Assays Chem 395 Spring 2007 Instructor : Dr. James Rusling Presenter : Bhaskara V. Chikkaveeraiah.

TECHNIQUES OF SYNTHESIZING CARBON NANOTUBE FETS FOR INTEGRATED CIRCUITS GAO, Feng S.I.D

Supplementary Fig.1: oligonucleotide primer sequences.

Application of Silicon Nanowire in Biosensor Student: HongPhan ID: Professor: Cheng-Hsien Liu.

Charge-Based Biosensor Using Carbon Nanotube Transistors Array Presenter: Jui-Ping Chiang.

Xlab.me.berkeley.edu Xlab Confidential – Internal Only EE235 Carbon Nanotube FET Volker Sorger.

NSF-ITR Meeting Miniaturized Sensors Air molecules aerosols Water molecules cells, viruses.

Colin Mann, Physics Professor Philip Collins ABSTRACT The use of carbon nanotube field-effect transistors (CNTFETs) as chemical or biological sensors is.

Electrochemical DNA Hybridization Sensors Shenmin Pan Bioanalytical Chemistry 395 Instructor: Prof. Rusling.

ELECTRICAL POROUS SILICON MICROARRAY FOR DNA HYBRIDIZATION DETECTION M. Archer*, D. Persaud**, K. D Hirschman**, M. Christophersen* and P. M Fauchet* *Center.

Carbon Nanotubes as Biosensors ME 695 Yang 11/30/2004.

Biosensors Christopher Byrd ENPM808B University of Maryland, College Park December 4, 2007.

Bio-detection using nanoscale electronic devices.

Class 7 FET, WGM and GMR based signal transduction

Lecture 19 OUTLINE The MOSFET: Structure and operation

D.L. Pulfrey Department of Electrical and Computer Engineering University of British Columbia Vancouver, B.C. V6T1Z4, Canada Carbon.

Brett Goldsmith, Ye Lu, Nicholas Kybert, A.T. Charlie Johnson University of Pennsylvania Department of Physics and Astronomy.

RFID Biosensors for the Detection of IEDs at 400 m Stand-off Distance

©2006 University of California Prepublication Data March 2006 In-situ Controlled Growth of Carbon Nanotubes by Local Synthesis Researchers Takeshi Kawano.

FET device and Molecular interaction 나노 물리소자 특강 Introduction Spring, 2008 Young June Park NSI_NCRC, Seoul National University Korea Nano Research Association.

Miniaturized Silicon Nanowire System for the In Vivo Diagnosis of Disease R. Myers, L. Rivera, Y. He, Z. Li and J. Villalba.

VFET – A Transistor Structure for Amorphous semiconductors Michael Greenman, Ariel Ben-Sasson, Nir Tessler Sara and Moshe Zisapel Nano-Electronic Center,

Low-cost organic gas sensors on plastic for distributed environmental sensing Vivek Subramanian Department of Electrical Engineering and Computer Sciences.

Microcantilevers III Cantilever based sensors: 1 The cantilever based sensors can be classified into three groups (i)General detection of any short range.

ICECS, Athens, December /18 From nanoscale technology scenarios to compact device models for ambipolar devices Sébastien Frégonèse, Cristell Maneux,

Applications of Aptamers as Sensors

Tamer Ragheb ELEC 527 Presentation Rice University 3/15/2007

M. Meyyappan Director, Center for Nanotechnology NASA Ames Research Center Moffett Field, CA 94035

Advisor : Ru-Li Lin Advisee :Shih-Min Chen Southern Taiwan University of Science and Technology, Department of Mechanical Engineering, Tainan, TAIWAN Date.

BioSensors Yang Yang 9/28/2004. Outlines BioMEMS Enzyme-coated carbon nanotubes Microcantilever biosensor with environmentally responsive hydrogel Cantilever.

IGZO thin film transistor biosensors functionalized with ZnO nanorods and antibodies Y.-C.Shen etal. / Biosensors and Bioelectronics 54 (2014) 306–310.

Scaling of the performance of carbon nanotube transistors 1 Institute of Applied Physics, University of Hamburg, Germany 2 Novel Device Group, Intel Corporation,

By Francesco Maddalena 500 nm. 1. Introduction To uphold Moore’s Law in the future a new generation of devices that fully operate in the “quantum realm”

1 Recent studies on a single-walled carbon nanotube transistor Reference ： (1) Mixing at 50GHz using a single-walled carbon nanotube transistor, S.Rosenblatt,

EE235 Presentation I CNT Force Sensor Ting-Ta YEN Feb Y. Takei, K. Matsumoto, I. Shimoyama “Force Sensor Using Carbon Nanotubes Directly Synthesized.

Carbon nanotube is a magic material. The unique structure brings it amazing characteristics. Lots of people believe that the usage of carbon nanotube will.

Biosensors and Carbon Nanotubes

POSTER TEMPLATE BY: Fabrication of Nanobiosensors Tom Fitzgerald, Nathan Howell, Brian Maloney Oregon State University, Department.

1 Carbon Nanotube In Biology Lawanya Raj Ojha Graduate Student Department of Chemistry, OSU, Stillwater.

Parametric Instability of Mobile Elastic Gate in Tera- and Nano- High Electron Mobility Transistor V.L. Semenenko, V.G. Leiman, A.V. Arsenin, A.D. Gladun.

Photo-Electronic Modulation of Nafion-gated Single-Walled Carbon Nanotube FETs Happiness Munedzimwe 3, S. V. Rotkin 1,2 1 Physics Department 2 Center for.

Slide # Goutam Koley Electronic characterization of dislocations MorphologyPotential 0.1 V /Div 10 nm /Div Surf. Potential G. Koley and M. G. Spencer,

Controlled Catalysis for CNT Growth at ARL Fe(NO 3 ) 3 Solution Catalyst Thin Metal Film Catalysts ~ 100  g Fe(NO 3 ) 3 /mL IPA ~ 25  g Fe(NO 3 ) 3 /mL.

March 3rd, 2008 EE235 Nanofabrication, University of California Berkeley Hybrid Approach of Top Down and Bottom Up to Achieve Nanofabrication of Carbon.

Nano and Giga Challenges in Microelectronics Symposium and Summer School Research and Development Opportunities Cracow Sep , 2004 Afternoon 4: Carbonanotubes.

Light sensors Chemical sensors Detection in liquids Biosensors Eye Nose Tongue Immune s. Converting molecular presence into an electronic signal.

Fatemeh (Samira) Soltani University of Victoria June 11 th

Analysis of Strain Effect in Ballistic Carbon Nanotube FETs

Photocurrent measurement in thin-film single-walled carbon nanotube field- effect transistors WEERAPAD DUMNERNPANICH FACULTY OF SCIENCE DEPARTMENT OF PHYSICS.

Graphene Transistors for Microwave Applications and Beyond Mahesh Soni1, Satinder Kumar Sharma1, Ajay Soni2 1School.

MoS2 RF Transistor Suki Zhang 02/15/17.

Riphah International University, Lahore

by Alexander Glavtchev

Carbon Nanotube Vias By: Rhesa Nathanael.

Power Dissipation in Nanoelectronics

Nanowire Gate-All-Around (GAA) FETs

Carbon Nanotubes Adam Charnas.

بیوسنسور (زیست حسگر) الهام سركي پاييز 1392.

Carbon Nanotube Diode Design

Subhayu Basu et al. , DNA8, (2002) MEC Seminar Su Dong Kim

Bio-detection using nanoscale electronic devices

Presentation transcript:

Label-Free Protein Biosensor Based on Aptamer-Modified Carbon Nanotube Field-Effect Transistors Kenzo Maehashi, Taiji Katsura, Kagan Kerman, Yuzuru Takamura, Kazuhiko Matsumoto, and Eiichi Tamiya Anal. Chem. 2007, 79, Jui-Ming (Ryan) Yang

Ryan Yang / Outline  Background CNT applications CNT biosensor : detection of Immunoglobulin E  Experimental Setup  Result  Conclusion / Consideration

Ryan Yang / Background  CNT – FET  CNT – Gas Sensor  CNT – Biosensor Protein Sensor DNA Sensor Lin, Y.-M., et al., IEEE Electron Device Lett. (2005) 26, 823 S Meng, et al. Nano Letter (2007) A Modi et al. (2003) Nature

Ryan Yang / Background  Goal: Detection of Immunoglobulin E (IgE)  Aptamer (synthetic oligonucleotides)  Monoclonal Antibody against IgE (IgE-mAb)  Debye Length: distance required for screening the surplus charge by mobile carriers present in the material

Ryan Yang / Experimental Setup  CNT-FET p+ type substrate / 100nm SiO2 source – drain contact: Ti/Au ethanol thermal CVD growth CNT  Custom-Synthesized DNA Aptamer  Immobilized Aptamer onto CNT 1-pyrenebutanoic acid succinimidyl ester with dry dimethylformamide solutions expose to aptamer in PBS overnight deactivate and block the excess reactive groups via ethanolamine D17.4ext: 5’-NH2-GCG CGG GGC ACG TTT ATC CGT CCC TCC TAG TGG CGT GCC CCG CGC-3’

Ryan Yang / Result  p-type characteristics in blank PBS  The current increased after the IgE- aptamer modification (at the source- drain bias of 0.2 V )  Stability test  Selectivity test Brovie serum albumin (BSA) binding event

Ryan Yang / Result  Sharp decrease in the source- drain current after introducing IgE, then saturation at lower values  Non-specific binding of IgE can be suppressed  Comparison to IgE-mAb modified CNT-FET

Ryan Yang / Conclusion  Aptamer modified CNT detects the immunoglobulin E with a good dynamic range, from 250pM to 20 nM  Promising candidate for the development of an integrated, high-throughput, real-time biosensor  Consideration Experimental setup scheme: gate reference Signal target detection limitation  Questions…